Agronomy and Horticulture Department

 

Date of this Version

2016

Citation

American Journal of Experimental Agriculture 11(3): 1-11, 2016, Article no.AJEA.20371

DOI: 10.9734/AJEA/2016/20371

Comments

© 2016 Al-Kaab et al.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License

Abstract

DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties. Seventy three primers out of 177 were reproducible and showed clear amplified bands. The degree of genetic diversity, Polymorphism information content (PIC) and resolving power (RP) were estimated. All the studied molecular markers were informative and showed good ability to classify and distinguish 16 wheat varieties. Total number of polymorphic bands is 134, 221 and 55 for ISSR, SRAP and RAPD respectively. PIC and RP values were 0.259, 0.264 and 0.262 and 9.06, 7.87 and 2.78