Staff Publications: Biological Process Development Facility Copyright (c) 2013 University of Nebraska - Lincoln All rights reserved. http://digitalcommons.unl.edu/bpdfpub Recent documents in Staff Publications: Biological Process Development Facility en-us Thu, 24 Jan 2013 12:39:22 PST 3600 A Timesaving Method for Labeling Figures, Tables and References http://digitalcommons.unl.edu/bpdfpub/7 http://digitalcommons.unl.edu/bpdfpub/7 Wed, 03 Oct 2012 13:53:20 PDT No matter how carefully an author outlines a manuscript, the figures, tables, and bibliographic citations are inevitably renumbered one or more times before the final draft. For example, the addition of a new reference to a late draft will sometimes entail the renumbering of all other references throughout the manuscript. A partial solution to this common technical problem in scientific writing is offered here. It is a refinement of an approach known to many scientists.

]]> Randy Carlson A PRESSURE INJECTION DEVICE FOR INOCULATION OF MAIZE WITH BACTERIAL PHYTOPATHOGENS http://digitalcommons.unl.edu/bpdfpub/6 http://digitalcommons.unl.edu/bpdfpub/6 Wed, 19 Sep 2012 08:47:00 PDT A pressure injection device (PID) was used to inoculate maize (Zea mays) with bacterial phytopathogens. The PID was evaluated for its speed, effectiveness, and precision relative to the needle-eye method of inoculation. Corynebacterium nebraskense, Erwinia stewartii, and Pseudomonas andropogonis were each used successfully as inocula to infect maize and produce symptoms typical of Goss's wilt, Stewart's wilt and bacterial stripe, respectively. Quantitative inoculation with the PID was faster and as statistically precise as using a calibrated needle-eye.

]]> Randall R. Carlson et al. LEAF SPOT OF FIELD CORN CAUSED BY <i>PSEUDOMONAS ANDROPOGONIS</i> http://digitalcommons.unl.edu/bpdfpub/5 http://digitalcommons.unl.edu/bpdfpub/5 Wed, 19 Sep 2012 08:22:08 PDT A leaf spot of field corn was shown to be caused by the bacterium Pseudomonas andropogonis. Leaf spot symptoms were observed over 3 years in several States. Inoculation of corn by vacuum infiltration of the bacteria was necessary to reproduce field symptoms.

A bacterial leaf spot disease of different cultivars of field corn was observed in early summer of 1973, 1974, and 1975. Leaves with essentially the same symptoms were obtained from South Dakota, Iowa, Kansas, Nebraska, and Michigan. The same symptoms were also seen in Wisconsin. Symptoms consisted of circular to ellipsoidal, tan to brown spots, with irregular margins. The spots were 1 to 4 mm in diameter, with one or more darker brown rings within the lesions. Some spots were surrounded by a chlorotic ring 1 mm wide. All spots tended to have a slightly sunken appearance. Occasionally the spots coalesced into irregular, somewhat elongated blotches. Water-soaking of young lesions was also seen. Bacteria were routinely isolated from the lesions. Because the symptoms did not correspond with previous reports of bacterial diseases of corn, experiments were undertaken to determine the causal bacterium and compare it with known bacterial pathogens of corn. The pathogen was subsequently identified as Pseudomonas andropogonis, previously reported to cause bacterial stripe disease of sorghum, sudangrass, teosinte, johnsongrass, field corn, broomcorn, and sweet corn. As P. stizolobii (syn. P. andropogonis), the bacterium causes leaf spot diseases of Bougainvillea, clover, and other leguminous plants.

]]> Anne M. Vidaver et al. Taxonomy of <i>Corynebacterium</i> Plant Pathogens, Including a New Pathogen of Wheat, Based on Polyacrylamide Gel Electrophoresis of Cellular Proteins http://digitalcommons.unl.edu/bpdfpub/4 http://digitalcommons.unl.edu/bpdfpub/4 Tue, 18 Sep 2012 09:03:53 PDT The known extant plant pathogenic Corynebacterium species were analyzed by polyacrylamide gel electrophoresis of their cellular proteins. The patterns of the protein bands of 13 species and a new corynebacterial wheat pathogen showed seven distinct groups. Five of these groups consisted of only one species each, one group contained four species, and the last group contained the new wheat pathogen and the remaining four species. The pathogens that could not be distinguished by the polyacrylamide gel analysis differed in phenotypic characteristics, including pathogenic specificity. Thus, for these bacteria we propose recognition of the following taxa: Corynebacterium fascians (Tilford) Dowson, Corynebacterium ilicis (Mandel et al.), Corynebacterium tritici (ex Hutchinson) nom. rev., Corynebacterium iranicum (ex Scharif) nom. rev., Corynebacterium rathayi (Smith) Dowson, Corynebacterium flaccumfaciens subsp. flaccumfaciens (Hedges) Dowson, Corynebacterium flaccumfaciens subsp. poinsettiae (Starr and Pirone) comb, nov., Corynebacterium flaccumfaciens subsp. betae (Keyworth et al.) comb, nov., Corynebacterium flaccumfaciens subsp. oortii (Saaltink and Maas Geesteranus) comb, nov., Corynebacterium michiganense subsp. michiganense (Smith) Jensen, Corynebacterium michiganense subsp. nebraskense (Schuster et al.) comb, nov., Corynebacterium michiganense subsp. insidiosum (McCulloch) comb, nov., Corynebacterium michiganense subsp. sepedonicum (Spieckermann and Kotthoff), comb, nov., and Corynebacterium michiganense subsp. tessellarius subsp. nov., the type strain of which is strain 78181 (= ATCC 33566 = PDDCC7221).

]]> Randall R. Carlson et al. Bacterial Mosaic, a New Corynebacterial Disease of Wheat http://digitalcommons.unl.edu/bpdfpub/3 http://digitalcommons.unl.edu/bpdfpub/3 Tue, 18 Sep 2012 07:39:40 PDT Bacterial mosaic is a foliar disease of wheat; characteristic symptoms are small yellow lesions, more or less uniformly distributed over the leaf. It was discovered in Nebraska in the spring of 1976. By 1979, the pathogen was distributed over an 800-km range and had been isolated from 16 cultivars of winter wheat. The pathogen is Corynebacterium michiganense subsp. tessellarius, a Gram-positive coryneform. Wheat was the only host that showed symptoms after inoculation. The pathogen reached high population levels (> 10' colony-forming units per gram of fresh weight) without apparent symptoms in tomato and in six of nine gramineous plants in greenhouse tests.

]]> Randall R. Carlson et al. MONOCLONAL ANTIBODY ASSAY AND KIT FOR DETECTING METAL CATIONS IN BODY FLUIDS (continuation) http://digitalcommons.unl.edu/bpdfpub/2 http://digitalcommons.unl.edu/bpdfpub/2 Mon, 17 Sep 2012 15:01:21 PDT The invention provides method and kits for detecting a metallic cation in a sample of a body fluid. The preferred method and kits include the use of at least two different types of antibodies having different specificities. In the preferred method, the sample of body fluid can be contacted with an effective amount of a capture antibody specific for a naturally occurring polypeptide that can bind the metallic cation to form a first antigen-antibody complex. An effective amount of an antibody specific for an epitope on a metallic cation-naturally occurring polypeptide complex or an antibody specific for a metallic cation is added to the first antigen-antibody complex to form a second antigen-antibody complex. The amount of the metallic cation in the sample of body fluid is determined by detecting the amount of the second antigen-antibody complex.

]]> Randall R. Carlson et al. MONOCLONAL ANTIBODY ASSAY AND KIT FOR DETECTING METAL CATIONS IN BODY FLUIDS http://digitalcommons.unl.edu/bpdfpub/1 http://digitalcommons.unl.edu/bpdfpub/1 Mon, 17 Sep 2012 14:57:35 PDT The invention provides method and kits for detecting a metallic cation in a sample of a body fluid. The preferred method and kits include the use of at least two different types of antibodies having different specificities. In the preferred method, the sample of body fluid can be contacted with an effective amount of a capture antibody specific for a naturally occurring polypeptide that can bind the metallic cation to form a first antigen-antibody complex. An effective amount of an antibody specific for an epitope on a metallic cation-naturally occurring polypeptide complex or an antibody specific for a metallic cation is added to the first antigen-antibody complex to form a second antigen-antibody complex. The amount of the metallic cation in the sample of body fluid is determined by detecting the amount of the second antigen-antibody complex.

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