Anuradha Subramanian
Associate Professor
Department of Chemical & Biomolecular Engineering
College of Engineering, University of Nebraska–Lincoln
207L OTHM Lincoln NE 68588-0643
asubramanian2@unl.edu

Research Interests:

Dr. Subramanian's interests are in the area of bioengineering, where engineering principles are called upon to solve applied problems in biological systems. Engineers now have the tools to design biological products and processes at the molecular level. The ability to manipulate the strength and specificity of protein-binding or cell-matrix binding events provides tremendous leverage for the development of novel biopharmaceuticals and biomolecularly-engineered surfaces. Dr. Subramanian is interested in protein adsorption at material surfaces in the area of bioseparations, and research currently involves the synthesis and characterization of zirconia aggregates with hierarchically structured pore architecture. Focus is placed on better understanding the mechanism of biomolecule transport as well as a fundamental investigation into the thermodynamics and the nature of affinity of these interactions.

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2011

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Impact of Porogens on the Pore Characteristics of Zirconia Particles Made by Polymer-Induced Colloid Aggregation, Abhinandan Pattanayak and Anuradha Subramanian

2008

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A Fundamental Study of the PCR Amplification of GC-rich DNA Templates, T. G. Mamedov, Elsje Pienaar, Scott E. Whitney, Joel R. TerMaat, G. Carvill, R. Goliath, A. Subramanian, and Hendrik J. Viljoen

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Control On Platelet Adhesion And Activation Events By Selective And Specific Binding Of Plasma Protein On Peptide-Coupled Surface, Anuradha Subramanian and Sanjukta Guha-Thankruta

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Importance of Annealing Times in the PCR Amplification of GC-Rich Genes, Anuradha Subramanian, Tarlan Mamedov, Elsje Pinnear, and Hendrik Viljoen

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Characterization of a Polymer Surface With Sequentially Immobilized Proteins, Anuradha Subramanian, Tarlan Mammedov, and Karl E. Kador

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Effect Of Intermittent Applications Of Continuous Ultrasound Signal On The Morphology And Cytoskeletal Organization In Chondrocytes, Anuradha Subramanian, Sandra Noriega, and Gulnara Hasanova

2007

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Detection of ultrathin biological films using vacuum ultraviolet spectroscopic ellipsometry, Dileep K. Goyal, Greg K. Pribil, John A. Woollam, and Anuradha Subramanian

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Effect of Hydrofluoric Acid in Oxidizing Acid Mixtures on the Hydroxylation of Silicon Surface, Sanjukta Guhathakurta and Anuradha Subramanian

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Rational de novo gene synthesis by rapid polymerase chain assembly (PCA) and expression of endothelial protein-C and thrombin receptor genes, Tarlan G. Mamedov, Nisha V. Padhye, Hendrik Viljoen, and Anuradha Subramanian

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Developement of serum-free media in CHO-DG44 cells using a central composite statistical design, Ananth Parampalli, Kent M. Eskridge, Leonard Smith, Michael M. Meagher, M. C. Mowry, and Anuradha Subramanian

2006

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A Comparative Study of Monoclonal Antibodies (Mabs) Purified from Cell Culture Supernatant on EDTPA-Modified Zirconia Beads and Protein A-Hyper D Support, Anuradha Subramanian, Blanca Martinez, Jill Holm, Peter W. Carr, and Clayton V. McNeff

Use of Modified Chitosan Macrospheres in the Selective Removal of Immunoglobulins, Anuradha Subramanian, Swapan Roy, Carmine Mascoli, and Jennifer Hommerding

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Use of a modified zirconia support in the separation of immunoproteins, Anuradha Subramanian and Sabyasachi Sarkar

2005

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UV-vis -infrared optical and AFM study of spin-cast chitosan films, W H. Nosal, D W. Thompson, Sabyasachi Sarkar, Anuradha Subramanian, J A. Woollam, and L Yan

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Identification of the Mass transfer mechanisms involved in the transport of human immunoglobulin - G in N,N,N',N' -ethylnediaminetetramenthylenephosphonic acid modified Zirconia, Sabyasachi Sarkar, Perer W. Carr, and Anuradha Subramanian

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Modeling of immunoglobulin uptake by N,N,N′,N′-ethylenediaminetetramethylenephosphonic acid-modified zirconia particles under static and dynamic conditions, Sabyasachi Sarkar and Anuradha Subramanian

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Chromatographic Purification of MAbs with Non-Affinity Supports, Anuradha Subramanian

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The use of confocal laser scanning microscopy to study the transport of biomacromolecules in a macroporous support, Anuradha Subramanian and Jennifer Hommerding

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Crosslinked chitosan: Its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation, Anuradha Subramanian and Hsin-YI Lin

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Preparation and evaluation of the electrospun chitosan/PEO fibers for potential applications in cartilage tissue engineering, Anuradha Subramanian, David Vu, Gustavo Larsen, and Hsin YIi Lin

2004

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Production and purification of a chimeric monoclonal antibody against botulinum neurotoxin serotype A , Mark C. Mowry, Michael Meagher LEAD INVESTIGATOR, Leonard Smith, and Anuradha Subramanian

2003

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Characterization and optimization of a chromatographic process based on ethylenediamine-N,N,N′,N′-tetra(methylphosphonic) acid-modified zirconia particles, Sabyasachi Sarkar, Peter W. Carr, Clayton V. McNeff, and Anuradha Subramanian

2000

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Immunoaffinity Chomatography, Anuradha Subramanian

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Purification of immunoglobulins from Serum Using Thiophilic Cellulose Beads, Anuradha Subramanian

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Ranking the Factors Impacting lmmunosorbent Performance, Anuradha Subramanian and William H. Velander

1999

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A Red-Dot-Blot Protein Assay Technique in the Low Nanogram Range, Tiilin Marcol and Anuradha Subramanian

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Bioactivities of a tumour necrosis like factor released by chicken macrophages, Silke Rautenschlein, Anuradha Subramanian, and Jagdev M. Sharma

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Method of coupling ligands onto supports and products produced therefrom , William H. Velander and Anuradha Subramanian

1996

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Effect of Antibody Orientation on Immunosorbent, Anuradha Subramanian and William H. Velander

1995

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Studies on plant resistance in sorghum to the chinch bug, {\it Blissus leucopterus leucopterus\/} (Say) (Hemiptera: Lygaeidae), Ramnath Subramanian

1993

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Investigating the role of market embeddedness in consumer outshopping behavior, Alka Subramanian

1992

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Consumer innovativeness as learned behavior: A cognitive social learning interpretation, Suresh Subramanian

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High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C, William H. Velander, John L. Johnson, Raymond L. Page, Chritopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittus, and William N. Drohan

1990

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Enzyme- Facilitated Transport and Separation of Organic Acids through Liquid Membranes, David C. Rethwisch, Anuradha Subramanian, Gao Yi, and Jonathan S. Dordick