Charles Bessey Professor of Chemistry
704 Hamilton Hall
dhage@unlserve.unl.edu

Research areas:
* Biochemical separations
* Bioanalytical chemistry
* Affinity chromatography
* High performance liquid chromatography
* Chromatographic immunoassays
* Biointeraction chromatography
* Drug-protein binding studies
* Biomolecule immobilization
* Monolithic columns

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2017

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Nanomaterials as Stationary Phases and Supports in Liquid Chromatography: A Review, Sandya Beeram, Elliott Rodriguez, Suresh Doddavenkatanna, Zhao Li, Allegra Pekarek, Darin Peev, Kathryn Goerl, Gianfranco Trovato, Tino Hofmann, and David S. Hage

2016

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Chromatographic Studies of Protein-Based Chiral Separations, Cong Bi, Xiwei Zheng, Shiden Azaria, Sandya Beeram, Zhao Li, and David S. Hage

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Optimizing Sequence Coverage for a Moderate Mass Protein in Nano-Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry, Ryan E. Matsuda, Venkata Kolli, Megan Woods, Eric D. Dodds, and David S. Hage

2014

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Analysis of biomolecular interactions using affinity microcolumns: A review, Xiwei Zheng, Zhao Li, Sandya Beeram, Maria Podariu, Ryan Matsuda, Erika L. Pfaunmiller, Christopher J. White II, NaTasha Carter, and David S. Hage

2013

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Effects of Fatty Acids and Glycation on Drug Interactions with Human Serum Albumin, Jeanethe Anguizola, Sara B. G. Basiaga, and David S. Hage

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Development of Affinity Microcolumns for Drug–Protein Binding Studies in Personalized Medicine: Interactions of Sulfonylurea Drugs with in vivo Glycated Human Serum Albumin, Jeanethe Anguizola, K. S. Joseph, Omar S. Barnaby, Ryan Matsuda, Guadalupe Alvarado, William Clarke, Ronald Cerny, and David S. Hage

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Review: Glycation of human serum albumin, Jeanethe Anguizola, Ryan Matsuda, Omar S. Barnaby, K. S. Hoy, Chunling Wa, Erin DeBolt, Michelle Koke, and David S. Hage

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Use of entrapment and high-performance affinity chromatography to compare the binding of drugs and site-specific probes with normal and glycated human serum albumin, Abby J. Jackson, Jeanethe Anguizola, Erika L. Pfaunmiller, and David S. Hage

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Studies of metabolite–protein interactions: A review, Ryan Matsuda, Cong Bi, Jeanethe Anguizola, Matthew Sobansky, Elliott Rodriguez, John Vargas Badilla, Xiwei Zheng, Benjamin Hage, and David S. Hage

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Affinity monolith chromatography: A review of principles and recent analytical applications, Erika L. Pfaunmiller, Marie Laura Paulemond, Courtney M. Dupper, and David S. Hage

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Analysis of free fractions for chiral drugs using ultrafast extraction and multi-dimensional high-performance affinity chromatography, Xiwei Zheng, Michelle J. Yoo, and David S. Hage

2012

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Pharmaceutical And Biomedical Applications Of Affinity Chromatography: Recent Trends And Developments, David S. Hage, Jeanethe A. Anguizola, Cong Bi, Rong Li, Ryan Matsuda, Efthimia Papastavros, John Vargas-Badilla, and Xiwei Zheng

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Analysis Of Drug Interactions With Modified Proteins By High-Performance Affinity Chromatography: Binding Of Glibenclamide To Normal And Glycated Human Serum Albumin, Ryan Matsuda, Jeanethe Anguizola, K.S. Joseph, and David S. Hage

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Optimization of human serum albumin monoliths for chiral separations and high-performance affinity chromatography, Erika L. Pfaunmiller, Mahli Hartmann, Courtney M. Dupper, Sony Soman, and David S. Hage

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Biointeraction Analysis of Immobilized Antibodies and Related Agents by High-Performance Immunoaffinity Chromatography, Erika L. Pfaunmiller, Annette C Moser, and David S. Hage

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IDENTIFICATION AND ANALYSIS OF STEREOSELECTIVE DRUG INTERACTIONS WITH LOW DENSITY LIPOPROTEIN BY HIGHPERFORMANCE AFFINITY CHROMATOGRAPHY, Matthew R. Sobansky and David S. Hage

2011

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COMPARISON OF MODIFICATION SITES FORMED ON HUMAN SERUM ALBUMIN AT VARIOUS STAGES OF GLYCATION, Omar S. Barnaby, Ronald Cerny, William Clarke, and David S. Hage

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QUANTITATIVE ANALYSIS OF GLYCATION PATTERNS IN HUMAN SERUM ALBUMIN USING 16O/18O-LABELING AND MALDI-TOF MS, Omar S. Barnaby, Ronald Cerny, William Clarke, and David S. Hage

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Bioanalysis Young Investigator Award – sponsored by Waters- Michelle J. Yoo, David S. Hage

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Chromatographic analysis of drug interactions in the serum proteome, David S. Hage, Jeanethe A. Anguizola, Abby J. Jackson, Ryan Matsuda, Efthimia Papastavros, Erika Pfaunmiller, Zenghan Tong, John Vargas-Badilla, Michelle J. Yoo, and Xiwei Zheng

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Characterization Of Drug Interactions With Serum Proteins by Using High-Performance Affinity Chromatography, David S. Hage, Jeanethe Anguizola, Omar S. Barnaby, Abby Jo Jackson, Michelle J. Yoo, Efthimia Papastavros, Erika Pfaunmiller, Matt Sobansky, and Zenghan Tong

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Research in bioanalysis and separations at the University of Nebraska – Lincoln, David S. Hage, Eric D. Dodds, Liangcheng Du, and Robert Powers

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BINDING OF TOLBUTAMIDE TO GLYCATED HUMAN SERUM ALBUMIN, K.S. Joseph, Jeanethe Anguizola, and David S. Hage

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HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND THE ANALYSIS OF DRUG INTERACTIONS WITH MODIFIED PROTEINS: BINDING OF GLICLAZIDE WITH GLYCATED HUMAN SERUM ALBUMIN, Ryan Matsuda, Jeanethe Anguizola, K.S. Joseph, and David S. Hage

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DEVELOPMENT OF A FLOW-BASED ULTRAFAST IMMUNOEXTRACTION AND REVERSE DISPLACEMENT IMMUNOASSAY: ANALYSIS OF FREE DRUG FRACTIONS, John E. Schiel, Zenghan Tong, Chainarong Sakulthaew, and David S. Hage

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CHARACTERIZATION OF INTERACTION KINETICS BETWEEN CHIRAL SOLUTES AND HUMAN SERUM ALBUMIN BY USING HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND PEAK PROFILING, Zenghan Tong and David S. Hage

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Detection of Heterogeneous Drug-Protein Binding By Frontal Analysis and High-Performance Affinity Chromatography, Zenghan Tong, K.S. Joseph, and David S. Hage

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KINETIC STUDIES OF DRUG-PROTEIN INTERACTIONS BY USING PEAK PROFILING AND HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY: EXAMINATION OF MULTI-SITE INTERACTIONS OF DRUGS WITH HUMAN SERUM ALBUMIN COLUMNS, Zenghan Tong, John E. Schiel, Efthimia Papastavros, Corey M. Ohnmacht, Quentin R. Smith, and David S. Hage

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Use of Peak Decay Analysis and Affinity Microcolumns Containing Silica Monoliths for Rapid Determination of Drug-Protein Dissociation Rates, Michelle J. Yoo and David S. Hage

2010

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QUANTITATIVE ANALYSIS OF GLYCATION SITES ON HUMAN SERUM ALBUMIN USING 16O/18O-LABELING AND MATRIXASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY, Omar S. Barnaby, Chunling Wa, Ronald Cerny, William Clarke, and David S. Hage

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Chromatographic Studies of Changes in Binding Of Sulfonylurea Drugs to Human Serum Albumin Due To Glycation and Fatty Acids, Sara B. G. Basiaga and David S. Hage

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Analysis Of Drug Interactions With High Density Lipoprotein By High-Performance Affinity Chromatography, Sike Chen, Matthew R. Sobansky, and David S. Hage

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Preparation of High-Capacity Supports Containing Protein G Immobilized To Porous Silica, Abby J. Jackson, Elizabeth M. Karle, and David S. Hage

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Entrapment of Proteins in Glycogen-Capped And Hydrazide-Activated Supports, Abby J. Jackson, Hai Xuan, and David S. Hage

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CHROMATOGRAPHIC ANALYSIS OF ACETOHEXAMIDE BINDING TO GLYCATED HUMAN SERUM ALBUMIN, K.S. Joseph, Jeanethe Anguizola, Abby J. Jackson, and David S. Hage

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Characterization of the Binding Of Sulfonylurea Drugs to HSA by High-Performance Affinity Chromatography, K.S. Joseph and David S. Hage

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The Effects of Glycation on the Binding of Human Serum Albumin to Warfarin and L-Tryptophan, K.S. Joseph and David S. Hage

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Analysis Of Drug-Protein Binding By Ultrafast Affinity Chromatography Using Immobilized Human Serum Albumin, Rangan Mallik, Michelle J. Yoo, Chad J. Briscoe, and David S. Hage

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Immunoaffinity chromatography: an introduction to applications and recent developments, Annette C Moser and David S. Hage

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Biointeraction analysis by high-performance affinity chromatography: Kinetic studies of immobilized antibodies, Mary Anne Nelson, Annette C. Moser, and David S. Hage

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Analysis of Lidocaine Interactions with Serum Proteins Using High-Performance Affinity Chromatography, Sony Soman, Michelle J. Yoo, Yoon Jeong Jang, and David S. Hage

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Biointeraction Analysis of Carbamazepine Binding To Alpha 1-Acid Glycoprotein by High-Performance Affinitychromatography, Hai Xuan, K. S. Joseph, Chunling Wa, and David S. Hage

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Evaluation of Affinity Microcolumns Containing Human Serum Albumin for Rapid Analysis of Drug-protein Binding, Michelle J. Yoo, John E. Schiel, and David S. Hage

2009

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Non-Competitive Peak Decay Analysis Of Drugprotein Dissociation By High-Performance Affinity Chromatography, Jianzhong Chen, John E. Schiel, and David S. Hage

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Evaluation of Indole-Based Probes for High throughput Screening Of Drug Binding To Human Serum Albumin: Analysis by High-Performance Affinity Chromatography, Mandi L. Conrad, Annette C Moser, and David S. Hage

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Characterization of Drug-Protein Interactions In Blood Using High-Performance Affinity Chromatography, David S. Hage, Abby Jackson, Matt Sobansky, John E. Schiel, Michelle J. Yoo, and K. S. Joseph

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Evaluation of Alternatives to Warfarin as Probes For Sudlow Site I of Human Serum Albumin Characterization by High-Performance Affinity Chromatography, K.S. Joseph, Annette C Moser, John E. Schiel, and David S. Hage

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Kinetic Studies of Biological Interactions By Affinity Chromatography, John E. Schiel and David S. Hage

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Measurement Of Drug-Protein Dissociation Rates By High-Performance Affinity Chromatography And Peak Profiling, John E Schiel, Corey M. Ohnmacht, and David S. Hage

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Evaluation of silica monoliths in affinity microcolumns for high-throughput analysis of drug–protein interactions, Michelle J. Yoo and David S. Hage

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Studies of Imipramine Binding To Human Serum Albumin By High-Performance Affinity Chromatography, Michelle J. Yoo, Quentin R. Smith, and David S. Hage

2008

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Development of an Affinity Silica Monolith Containing Human Serum Albumin For Chiral Separations, Rangan Mallik and David S. Hage

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Immobilization Of Alpha 1-Acid Glycoprotein For Chromatographic Studies Of Drug-Protein Binding Ii. Correction For Errors In Association Constant Measurements, Rangan Mallik, Hai Xuan, Georges Guiochon, and David S. Hage

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Studies of Verapamil Binding to Human Serum Albumin By High-Performance Affinity Chromatography, Rangan Mallik, Michelle J. Yoo, Sike Chen, and David S. Hage

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Capillary Electrophoresis-Based Immunoassays: Principles & Quantitative Applications, Annette C Moser and David S. Hage

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Estimating Protein-Ligand Binding Affinity using High- Throughput Screening by NMR, Matthew D. Shortridge PhD, David S. Hage, Gerard S. Harbison, and Robert Powers

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Determination of Free Catecholamines in Urine By Tandem Affinity/Ion-Pair Chromatography and Flow Injection Analysis, David H. Thomas, Jay D. Taylor, Omar S. Barnaby, and David S. Hage

2007

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Development Of Sulfhydryl-Reactive Silica For Protein Immobilization In High-Performance Affinity Chromatography, Rangan Mallik, Chunling Wa, and David S. Hage

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Development Of An Affinity Silica Monolith Containing α1- Acid Glycoprotein For Chiral Separations, Rangan Mallik, Hai Xuan, and David S. Hage

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Environmental Analysis By On-Line Immunoextraction And Reversed-Phase Liquid Chromatography: Optimization Of The Immunoextraction/RPLC Interface, Mary Anne Nelson, Efthimia Papastavros, Maud Dodlinger, and David S. Hage

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Characterization of Glycation Adducts On Human Serum Albumin by Matrix Assisted Laser Desorption/ Ionization Time-Of-Flight Mass Spectrometry, Chunling Wa, Ronald Cerny, William A. Clarke, and David S. Hage

2006

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Quantitative Studies of Allosteric Effects By Biointeraction Chromatography: Analysis of Protein Binding For Low Solubility Drugs, Jianzhong Chen and David S. Hage

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Chromatographic analysis of carbamazepine binding to human serum albumin: II. Comparison of the Schiff base and N-hydroxysuccinimide immobilization methods, Hee Seung Kim, Rangan Mallik, and David S. Hage

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Stability of warfarin solutions for drug–protein binding measurements: Spectroscopic and chromatographic studies, Annette C. Moser, Charles A. Kingsbury, and David S. Hage

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Studies by biointeraction chromatography of binding by phenytoin metabolites to human serum albumin, Corey M. Ohnmacht, Shirley Chen, Zenghan Tong, and David S. Hage

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Analysis of Free Drug Fractions Using Near-Infrared Fluorescent Labels and an Ultrafast Immunoextraction/Displacement Assay, Corey M. Ohnmacht, John E. Schiel, and David S. Hage

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Identification and Quantitative Studies of Protein Immobilization Sites by Stable Isotope Labeling And Mass Spectrometry, Chunling Wa, Ronald Cerny, and David S. Hage

1999

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Affinity Chromatography: A Review of Clinical Applications, David S. Hage

1992

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Intact Parathyroid Hormone: Performance and Clinical Utility of an Automated Assay Based on High-Performance Immunoaffinity Chromatography and Chemiluminescence Detection, David S. Hage, Bob Taylor, and Pai C. Kao

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Improved Recovery of a Radlolabeled Peptide with an Albumin-Treated Reversed-Phase HPLC Column, David S. Hage, Robert L. Taylor, and Pai C. Kao

1991

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Use of Affinity Chromatography in Developing Acridinium Ester-Labeled Antibodies for an Immunometric Assay of Parathyrin, David S. Hage, Bob Taylor, Pat Schryver, and Pai C. Kao