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Genetic variation of the European corn borer (Ostrinia nubilalis) (Hubner) Lepidoptera: Crambidae)

Lilian Aguiar Saldanha, University of Nebraska - Lincoln

Abstract

Genetic variation of European corn borer (Ostrinia nubilalis (Hiibner)) ecotypes from Nebraska populations was evaluated using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). After consulting the literature and screening several primers, two RAPD primers (OPF-03 and OPG-03) were used to assess the genetic variation of O. nubilalis from five sites in Nebraska. The data analyzed included only visible and reproducible band fragments from two agreeing gels both of which were reproducible across DNA samples from insects of known ecotype. Twenty-five characters resulted and were scored from the two primers. They were coded as vectors of 1s and 0s, each representing band presence or absence, respectively. The pairwise similarities of the profiles were computed using the algorithm of Jaccard and Nei and Li, which were then used in Neighbor and UPGMA (unweighted pair-group mean averages) to determine the affinity among populations using the Neighbor program in PHYLIP (Phylogeny Inference Package). The Neighbor dendrogram indicates that O. nubilalis populations in Nebraska consisted of 35.5% univoltine, 44.5% bivoltine, and 20% multivoltine ecotypes. These results showed that O. nubilalis from Nebraska constituted a broad genetic group. ^ The inheritance of RAPD fragments generated via polymerase chain reaction (PCR) from genomic DNA of parents and offspring of O. nubilalis in four families of bivoltine, univoltine, multivoltine, and bivoltine/univoltine (laboratory hybrids) ecotypes was investigated. Parents and twenty-four individuals of F2 progeny were scored for the segregation of fragments generated with nine RAPD primers. A total of 139 amplified fragments were obtained. For the primers investigated, 57 fragments revealed a 1:1 segregation ratio, whereas 33 fragments showed a 3:1 segregation ratio, determined by Chi-square at the 5% level of probability. In contrast, 28 fragments present in both parents revealed no segregation in the progenies. This study demonstrates the suitability of the PD primers to assess the inheritance patterns of RAPD primers. However, parental determination may not be recommended because of the appearance of novel RAPD-PCR amplified fragments of the progeny that were not present in the parents. ^

Subject Area

Biology, Molecular|Biology, Entomology

Recommended Citation

Saldanha, Lilian Aguiar, "Genetic variation of the European corn borer (Ostrinia nubilalis) (Hubner) Lepidoptera: Crambidae)" (2000). ETD collection for University of Nebraska - Lincoln. AAI9992007.
http://digitalcommons.unl.edu/dissertations/AAI9992007

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