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<title>Nutrition and Health Sciences -- Faculty Publications</title>
<copyright>Copyright (c) 2013 University of Nebraska - Lincoln All rights reserved.</copyright>
<link>http://digitalcommons.unl.edu/nutritionfacpub</link>
<description>Recent documents in Nutrition and Health Sciences -- Faculty Publications</description>
<language>en-us</language>
<lastBuildDate>Thu, 24 Jan 2013 19:55:29 PST</lastBuildDate>
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<title>In-home Prenatal Nutrition Intervention
Increased Dietary Iron Intakes and
Reduced Low Birthweight in
Low-Income African-American
Women</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/18</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/18</guid>
<pubDate>Wed, 16 May 2012 15:10:44 PDT</pubDate>
<description>
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	<p>Low birthweight (LBW), defined as an infant weighing less than 2,500 grams (1), is a major determinant of infant mortality and contributes to infant and childhood morbidity and increased healthcare costs (2-4). LBW occurs due to preterm delivery (length of gestation less than 37 weeks), growth retardation, or both (2). In the United States, higher rates of LBW occur in urban areas with high concentrations of low-income ethnic minorities (5). Results of the 1997 Pediatric Nutrition Surveillance indicate that the rate of LBW in the United States was 13% for African-American infants, and 8% for whites (6). Healthy People 2010 (7) outlines an overarching goal to eliminate health disparities among different segments of the population, including differences that occur by race or ethnicity</p>
<p>Reviews of the medical nutrition therapy literature indicate a continuing need to document the specific contribution of the registered dietitian to health outcomes attained (8). In the specific area of prenatal nutrition interventions, Boyd and Windsor (9) concluded there is a need for high-quality prenatal nutrition education programs, including descriptions of program process and content. Published reports of nutrition interventions suggest that programs are more likely to be successful if they are adapted to the particular minority target culture (10) and if they are individualized to the specific client’s needs (11). A continuing need exists for well-documented and effective prenatal nutrition interventions designed to reduce the incidence of LBW among minority populations. The objective of this project was to evaluate an in-home prenatal nutrition intervention designed to prevent LBW among low-income African-American women.</p>

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<author>Chiquita Briley et al.</author>


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<title>Constructive Developmental Theory:
An Alternative Approach to Leadership</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/17</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/17</guid>
<pubDate>Fri, 11 May 2012 12:02:39 PDT</pubDate>
<description>
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	<p>As early as 1954, the critical value of leadership in dietetics was described by Rourke, who wrote, “When the day comes that your executive abilities equal your scientific knowledge, your profession (dietetics) will be secure. Until that day, you will be faced with a constant and unwelcome challenge” (1). Career and leadership skills development have been identified as an area for further attention by American Dietetic Association (ADA) practitioners and student members (2). Creation of the ADA Leadership Institute in 2003 represented an awareness of the need to invest in developing dietetic leaders (3). Proficient leaders are critical in keeping our profession on the cutting edge by identifying areas of need for change and providing leadership for change. Proficient leaders can also serve as role models for members.</p>
<p>Leadership has been identified as essential for success in the 21st century and according to Bennis, “our quality of life amidst the volatility, turbulence and ambiguity of our present day societal context, depends on the quality of our leaders” (4). Leadership research theory has been based on objective measures such as traits, attitudes and performance, intellect, personality, relationships, competencies, and values. Dietetic leadership studies are limited and describe these objective characteristics and leadership styles (5-9). In their review of traditional leadership theory, Gregoire and Arendt (10) suggested that more information is needed about how dietitians develop as leaders. The present article describes one theory of leadership development—constructive developmental theory. Registered dietitians at advanced leadership stages can be identified and factors enabling their development can be studied. This theory provides a subjective approach for studying dietetic leadership and gives insight for leadership development programs.</p>

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<author>Anne Marie B. Hunter et al.</author>


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<title>Serum Lipid Response to n-3 Fatty Acid Enriched Eggs in Persons with Hypercholesterolemia</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/16</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/16</guid>
<pubDate>Fri, 11 May 2012 09:35:19 PDT</pubDate>
<description>
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	<p>Consumption of long-chain n-3 polyunsaturated fatty acids (n-3 PUFA) is low among certain population subgroups in the United States, especially in people living in Midwestern states and those who dislike fish. Therefore, there is a need to identify acceptable food sources of n-3 PUFA. Vegetable oils, such as canola and soy, provide some n-3 fatty acids. However, these sources are high in n-6 fatty acids and there is concern that they may have detrimental side effects. Eggs enriched in n-3 PUFA can be produced by incorporating sources of these fatty acids into poultry rations. In acceptability studies, US consumers responded positively to n-3 enriched eggs. However, Americans may be reluctant to consume eggs as a source of n-3 PUFA because of their cholesterol content.</p>
<p>The purpose of this study was to evaluate the effects of the addition of LNA- and DHA-enriched eggs on serum lipids of people with hyperlipidemia who were consuming a low-fat diet.</p>

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<author>Nancy M. Lewis et al.</author>


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<title>Validity and Reliability of a Quantitative Food Frequency Questionnaire Measuring n-3 Fatty Acid Intakes in Cardiac Patients in the Midwest: A Validation Pilot Study</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/15</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/15</guid>
<pubDate>Wed, 10 Aug 2011 07:48:11 PDT</pubDate>
<description>
	<![CDATA[
	<p>The purpose of this pilot study was to test the validity and reliability of a quantitative n-3 fatty acid food frequency questionnaire (FFQ) for later use with larger groups of individuals. A convenience sample of heart patients provided dietary data via three 24-hour food recalls and FFQs. Participants were women (n = 17) and men (n = 11), 43 to 77 years of age. The association of mean daily intake of n-3 fatty acids obtained using food recalls and the FFQ was assessed by Pearson correlation. The reliability of the FFQ was assessed using coefficient α. Correlation of n-3 fatty acid intake using the food recalls and the FFQ was r = 0.42 (P < 0.05). The coefficient α for the test-retest of the FFQ was .83. The top two foods, walnuts and flaxseed, contributed 58% of the n-3 fatty acid intake, and the third food, salmon, contributed 5%. This quantitative n-3 FFQ is a valid instrument for use in place of food recalls for estimating n-3 fatty acid intakes in heart patients and is a reliable instrument to estimate n-3 fatty acid intakes from plant, animal, and seafood sources. The FFQ should be tested in a larger population. Registered dietitians can use this FFQ to screen for intakes, educate patients on food sources, and measure change in intakes after nutrition intervention.</p>

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<author>Paula K. Ritter-Gooder et al.</author>


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<title>Clinical Registered Dietitians, Employers, and Educators Are Interested in Advanced Practice Education and Professional Doctorate Degrees in Clinical Nutrition</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/14</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/14</guid>
<pubDate>Wed, 10 Aug 2011 07:21:36 PDT</pubDate>
<description>
	<![CDATA[
	<p>A subset of registered dietitians (RDs) is known to practice at an advanced level, but a clear educational pathway supporting advanced medical nutrition therapy practice has not been identified. Thus, an electronic survey was designed to investigate interest of clinical RDs, employers, and educators in advanced practice competencies and professional doctorate degree programs in clinical nutrition. Usable responses were obtained from 440 of 978 (45%) RDs, 61 of 107 (57%) employers, and 76 of 114 (67%) educators. Mean interest (5 = very interested, 1 = very uninterested) in obtaining advanced practice education was highest among RDs (3.93 ±1.01) and was significantly different (P < 0.01) from employers (3.74 ±1.28) and educators (2.76 ±1.33). Interest in completing a professional doctorate in clinical nutrition was significantly (P < 0.01) different among RDs (3.05 ±1.28), employers (3.18 ±1.30), and educators (2.3 ±1.34). Employers’ mean interest score for hiring RDs with a professional doctorate in clinical nutrition was 4.02 ±0.93. A subset of clinical RDs appears to be interested in obtaining advanced practice competency and enrolling in professional doctorate degrees in clinical nutrition. Clinical nutrition managers in academic medical centers may be interested in hiring advanced practice clinical RDs with professional doctorate degrees. Opportunities exist for educators to develop advanced practice educational experiences and professional doctorate degree programs.</p>

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<author>Annalynn Skipper et al.</author>


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<title>Using Initiative to Achieve Autonomy: A Model for Advanced Practice in Medical Nutrition Therapy</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/13</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/13</guid>
<pubDate>Fri, 08 Jul 2011 12:33:31 PDT</pubDate>
<description>
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	<p>The purpose of this research was to generate a model of advanced medical nutrition therapy (MNT) practice based on descriptions of their clinical activities provided by advanced- level MNT practitioners. A qualitative approach using grounded theory methodology was employed. The model of advanced MNT practice was based on interviews with 21 credentialed advanced practice registered dietitians from across the United States and Canada. Verbatim transcripts of the interviews were coded for aggregation into themes and subthemes. The resulting model included a single overarching theme: using initiative to achieve autonomy. The five subthemes are aptitude, attitude, expertise, context, and approach. The model provides a richer picture of advanced MNT practice than was previously available and is presented for discussion and debate. Validation of the model is required before incorporation into education or practice.</p>

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<author>Annalynn Skipper et al.</author>


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<title>Content Validity of Nutrition Diagnostic Term Involuntary Weight Loss</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/12</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/12</guid>
<pubDate>Tue, 05 Jul 2011 08:43:39 PDT</pubDate>
<description>
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	<p><strong>Objective </strong>The purpose of this study was to validate content of the Nutrition Diagnostic Term NC- 3.2 Involuntary Weight Loss using expert raters.</p>
<p><strong>Design/Subjects</strong> This descriptive survey invited all Board Certified Specialists in Gerontological Nutrition (CSG) to participate by mail. An instrument was developed that included the definition, etiologies, and signs and symptoms (s/s) of the diagnosis with items added from literature review. CSG rated how common or characteristic each item is to the diagnosis using a 5 point Likert scale. A weighted response for each item was used to calculate a Diagnostic Content Validity (DCV) score. DCV scores of 0.80 and above were classified as major characteristics, 0.50 to 0.799 were minor characteristics, and those scoring below 0.50 were unrepresentative of the diagnosis. A mean total DCV score was calculated using the major and minor characteristics. Dietitians were asked to comment on clarity and completeness of the language.</p>
<p><strong>Results/Discussion </strong>Seventy three percent of CSG (n=110) had participated, 43% percent had an MS degree or higher level of education. Reported years of practice in gerontological nutrition were 15±10 years (mean ± SD). The DCV score was 0.80 for the definition, 0.63 for the etiologies, and 0.70 for s/s. Total DCV score for the diagnostic term was 0.69. Thirty six percent and 40% of the CSG recommended adding language to etiologies and s/s respectively. Results indicate the majority of items were valid to the diagnostic term but responses for adding items need further investigation.</p>

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<author>Paula K. Ritter-Gooder et al.</author>


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<title>Development and pilot testing of an omega-3 fatty acid food frequency questionnaire</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/11</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/11</guid>
<pubDate>Tue, 05 Jul 2011 08:41:00 PDT</pubDate>
<description>
	<![CDATA[
	<p>Omega-3 fatty acids are essential to health and provide cardio-protective benefits. To estimate omega-3 fatty acid dietary intake, we developed a semi-quantitative food frequency questionnaire (FFQ) and pilot tested its reliability and validity in a convenience sample of cardiac patients in the Midwest. The FFQ was developed using the USDA Nutrient Database for Standard Reference and the ESHA Food Processor, ESHA Research, Salem, Oregon. Three 24-h recalls and two administrations of the FFQ two weeks apart were obtained by a dietitian by phone and home visit from 28 volunteers. Pearson correlation was used to assess the association between food recalls and FFQ. Alpha coefficient was used to assess reliability of the FFQ. The correlation of omega-3 fatty acids intake using the food recalls and FFQ was 0.42 (P<0.05). Reliability of the FFQ was 0.83 with the food categories ranging from 0.93 for grains to 0.57 for vegetables. Ninety percent of omega-3 fatty acid intake was provided by 28 foods, with the top two food sources from plant origin. The FFQ yielded excellent reliability and acceptable validity in the population tested and can be used to estimate total omega-3 fatty acid intake from plant, animal, and seafood sources in the diets of cardiac patients.</p>

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<author>Paula K. Ritter-Gooder et al.</author>


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<title>Omega-3 Fatty Acid Consumption and Food Sources Differ among Elderly Men Living in Coastal and Internal Regions of Saudi Arabia</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/10</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/10</guid>
<pubDate>Wed, 29 Jun 2011 09:10:30 PDT</pubDate>
<description>
	<![CDATA[
	<p>Research suggests that omega-3 fatty acids (n-3 FAs) play an important role in reducing the risk of heart diseases. The objectives of the current study were to investigate the consumption and the food sources of n-3 FAs in two samples of elderly men living in different geographic locations (coastal and internal regions) of Saudi Arabia. Sixty men from each of the coastal and the internal regions provided a 24-hour recall and 2-day food record and a food frequency questionnaire. Food Processor Plus computer program software (ESHA Version 0.7; Salem, Oregon, 2002) was used to analyze the dietary intake. The intake of total n-3 FAs, alpha-lenolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were higher among the coastal region residents than among the internal residents (P < 0.05). English walnuts, salmon, canola oil, malabar cavalla and king mackerel were the top five foods contributing to the n-3 FA intakes in the coastal region. The top five foods contributing to the n-3 FA intakes in the internal region were English walnuts, lamb, whole milk, baked beans and chicken. The food contributing the most to the intake of n-3 FAs in both regions were English walnuts. In conclusion, the food consumption pattern for food providing n-3 FAs differs by location and coastal residents consume more than twice as much n-3 FAs as internal residents. Nutrition education intervention among internal residents is needed for increasing the consumption of n-3 FAs.</p>

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<author>Khalid AL-Numair et al.</author>


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<title>Validity and Reliability of an Omega-3 Fatty Acid Food Frequency Questionnaire for First-Generation Midwestern Latinas</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/9</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/9</guid>
<pubDate>Wed, 29 Jun 2011 08:40:34 PDT</pubDate>
<description>
	<![CDATA[
	<p>This study tested the hypothesis that a culturally developed omega-3 (n-3) fatty acid food frequency questionnaire (FFQ) could be an accurate instrument to capture n-3 fatty acid food intakes of first-generation Midwestern Latinas. The goal of the study was to assess validity and test-retest reliability of an FFQ to estimate total n-3 fatty acid (total n-3), α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) intakes. An n-3 FFQ was developed and pilot tested. Two FFQs and 3 nonconsecutive 24-hour recalls were collected from 162 participants. Pearson correlation and paired t test were used to test the hypothesis. Correlation of the 2 FFQs was 0.71 for total n-3, 0.65 for ALA, 0.74 for EPA, and 0.54 for DHA (P < .01). The means of the 2 FFQs and of the 24-hour recalls were not significantly different for total n-3 and ALA (P > .05), but were significantly different for EPA and DHA. The n-3 FFQ had acceptable reliability, validated only total n-3 and ALA, and provided relevant findings about the n-3 eating habits of Midwestern Latinas</p>

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<author>Karina R. Lora et al.</author>


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<title>Research Methodology for Validation of Nutrition Diagnostic Standardized Language Using Fehring Models</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/8</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/8</guid>
<pubDate>Thu, 23 Jun 2011 08:46:07 PDT</pubDate>
<description>
	<![CDATA[
	<p>Validation of the nutrition standardized language (SL) confirms the language is accurate for use in practice, research, and policy. Nutrition diagnostic terms are identified by concept analysis using literature review and experts to distinguish and differentiate one nutrition problem from a similar or related problem. The Fehring models, used in nursing diagnosis validation research, are adopted to validate the SL nutrition diagnostic terms using experts and weighted inter-rater reliability ratios. In the first phase of validation research, diagnostic terms are validated by experts using the Diagnostic Content Validity Model (DCV). A Nutrition Diagnosis Validation Instrument (NDVI) is developed that contains the definition, etiologies, and signs and symptoms (s/s) of the diagnosis plus items added from literature review. Questions regarding clarity and completeness of the term are included. The NDVI uses a Likert-type scale with assigned weights to derive a DCV score. Using the weighted mean score, each item is classified into major (≥ 0.80), minor (0.50 to 0.79), or non- relevant (below 0.49) characteristics. The Clinical Diagnostic Validation Model (CDV) is employed in the second phase to validate the term in the clinical setting observing patients with the nutrition problem using the NDVI, expert dietitians and a CDV score. Use of the DCV and CDV Models provides clear, comprehensive and observable lists of defining s/s needed for accurate and valid SL diagnostic terms and allows comparisons between studies.</p>

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<author>Paula K. Ritter-Gooder et al.</author>


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<title>Validation of Nutrition Standardized Language—Next Steps</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/7</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/7</guid>
<pubDate>Thu, 23 Jun 2011 08:39:57 PDT</pubDate>
<description>
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	<p>To provide a model for quality care and outcomes management, the Nutrition Care Process (NCP) and standardized language were introduced in 2003 by the American Dietetic Association (ADA). The standardized language, published in the <em>International Dietetics & Nutrition Terminology (IDNT) Reference Manual: Standardized Language for the Nutrition Care Process</em>, is designed to facilitate communication, improve care, and close the gap in health care quality. When registered dietitians (RDs) are oriented to a nutrition care process, improvement in their documentation of nutrition care occurs. Since the introduction of the NCP and standardized language, RDs and dietetic technicians, registered (DTRs), have begun to adopt the process and language into their practice. This article explains why validation of the standardized language is needed to make the language accurate and meaningful for practice and describes how validation is conducted using RDs.</p>

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<author>Paula Ritter-Gooder et al.</author>


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<title>Content Validation of a Standardized Language Diagnosis by Certified Specialists in Gerontological Nutrition</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/6</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/6</guid>
<pubDate>Tue, 14 Jun 2011 09:20:37 PDT</pubDate>
<description>
	<![CDATA[
	<p>Validation of the nutrition standardized language assures the language is accurate for use in practice, policy, and research, but few validation studies have been reported. The purpose of this descriptive study was to validate content of all components of the nutrition diagnostic term involuntary weight loss using experts providing care for older adults in health care settings. A Nutrition Diagnosis Validation Instrument was developed that contained the definition, etiologies, and signs and symptoms of the diagnosis plus items added from literature review. Questions on clarity and completeness of the language were included. The Nutrition Diagnosis Validation Instrument used a Likert-type scale for deriving a Diagnostic Content Validity (DCV) score for all items in the definition, etiology, and signs and symptoms components to define major, minor, and nonrelevant characteristics and a mean total DCV score for the term. In 2008, all Board Certified Specialists in Gerontological Nutrition (CSGs) were recruited by mail. CSGs (n = 110, 73% response) reported 15 ± 10 (mean ± standard deviation) practice years in gerontological nutrition. The total DCV component scores were 0.80 ± 0.17 (definition), 0.63 ± 0.08 (etiology), and 0.69 ± 0.12 (signs and symptoms). The mean total DCV score of the diagnostic term was 0.69 ± 0.11. Cognitive decline, poor oral health, and impaired skin integrity were identified as missing language. In conclusion, the majority of the definition, etiologies, and signs and symptoms of the term were contentvalidated, including seven items derived from literature review. The validated items, including recommendations for added language, need to be retested using the same process.</p>

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<author>Paula K. Ritter-Gooder et al.</author>


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<title>Correlation of Omega-3 Fatty Acids Intakes with Acculturation and Socioeconomic Status in Midwestern Latinas</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/5</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/5</guid>
<pubDate>Tue, 14 Jun 2011 08:01:49 PDT</pubDate>
<description>
	<![CDATA[
	<p><strong>Background:</strong> Low socioeconomic status (SES) and acculturation of Latino immigrants in the U.S. are linked to a decrease in diet quality.<strong> Methods:</strong> Interviews were conducted with 162 first-generation Latinas to examine the association of SES and acculturation with intake of omega-3 (<em>n</em> − 3) fatty acids. Each participant provided dietary intake by use of a validated <em>n</em> − 3 food frequency questionnaire administered twice, 4 weeks apart, three 24-h recalls, sociodemographic information and completed the 5-item Short Acculturation Scale. <strong>Results:</strong> Mean intakes of Total <em>n</em> − 3, α-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (g/d) were 1.2 ± 0.7, 1.1 ± 0.6, and 0.1 ± 0.1, respectively. After adjusting for energy intake, education was significantly correlated with EPA + DHA intakes, and acculturation was significantly correlated with Total <em>n</em> − 3, ALA and EPA + DHA intakes. Foods sources of EPA + DHA eaten by at least 50% of participants were chicken, shrimp, tuna and eggs. <strong>Discussion:</strong> Given the beneficial cardiovascular effects of<em> n</em> − 3 fatty acids, it is important to understand sociocultural factors affecting adequate intake towards an improvement in diet quality in minorities.</p>

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<author>Karina R. Lora et al.</author>


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<title>Survey of Omega-3 Fatty Acids in Diets of Midwest Low-Income Pregnant Women</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/4</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/4</guid>
<pubDate>Mon, 13 Jun 2011 13:46:33 PDT</pubDate>
<description>
	<![CDATA[
	<p>The objectives of this project were to detennine the omega-3 fatty acid (ω-3 FA) consumption of low-income pregnant women, and to identify food sources of ω-3 FA in their diets. Thirty women provided three days of dietary intake that were analyzed using the Food Processor Plus computer program. Mean daily consumption of ω<em>-3 </em>FA was 1.060 ± 0.030 g/day. The major form of the ω<em>-3 </em>FA was α-linolenic acid (α-LNA; 93%), with less from eicosapentanoic acid (EPA; 2%), and docosahexanoic acid (DHA; 5%). Foods that provided the most α-LNA were fats, oils, salad dressings, and milk products. EPA and DHA were provided in fish, seafood, chicken, and eggs. Nearly one-half of the women in this study consumed < 75% of the Canadian Recommended Nutrient Intakes for ω<em>-3 </em>FA. We conclude that some low-income pregnant women in the Midwest consume diets low in ω<em>-3 </em>FA. There is a need for agriculture and nutrition research on the development of new food products that provide ω<em>-3 </em>FA.</p>

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<author>Nancy M. Lewis et al.</author>


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<title>Enriched Eggs as a Source of N-3 Polyunsaturated Fatty Acids for Humans</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/3</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/3</guid>
<pubDate>Mon, 13 Jun 2011 13:39:46 PDT</pubDate>
<description>
	<![CDATA[
	<p>Dietary intake of omega-3 fatty acids (n-3 PUFA) decreases the risk of heart disease, inhibits the growth of prostate and breast cancer, delays the loss of immunological functions, and is required for normal fetal brain and visual development. The US has not established a recommended daily intake for n-3 PUFA. However, Canada has established the Canadian Recommended Nutrient Intake (CRNI) at 0.5% of energy. Dietary sources of n-3 PUFA include fish, chicken, eggs, canola oil, and soybean oil. Food consumption studies in the US indicate that the majority of Americans do not meet the CRNI for n-3 PUFA. Mean n-3 PUFA consumption was 78% of the CRNI for Midwestern women during pregnancy. In Midwestern women at risk for breast cancer, the mean n- 3 PUFA consumption is approximately 50% of the CRNI. Increased consumption of n-3 PUFA requires identification of a food source that the public would eat in sufficient amounts to meet recommended intake. N-3 PUFA-enriched eggs can be produced by modifying hens diets. When 70 g/kg of cod liver oil, canola oil, or linseed oil are added to a commercial control diet, the n-3 PUFA are increased from 1.2% of egg yolk fatty acids to 6.3, 4.6, and 7.8%, respectively. Feeding flaxseed increases linolenic acid in the egg yolk about 30-fold, and docosahexaenoic acid (DHA) increases nearly fourfold. When individuals are fed four n-3 PUFA-enriched eggs a day for 4 wk, plasma total cholesterol levels and low-density lipoprotein cholesterol (LDL-C) do not increase significantly. Plasma triglycerides (TG) are decreased by addition of n-3 PUFA-enriched eggs to the diet. N-3 PUFA may influence LDL particle size, causing a shift toward a less atherogenic particle. Blood platelet aggregation is significantly decreased in participants consuming n-3 PUFAenriched eggs. Overall results of studies to date demonstrate positive effects and no negative effects from consumption of n-3-enriched eggs. Three n-3 PUFA-enriched eggs provide approximately the same amount of n-3 PUFA as one meal with fish. It is recommended that n- 3 PUFA-enriched eggs be used as one source of n-3 PUFA to increase individual consumption to meet the current Canadian recommendations.</p>

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<author>Nancy M. Lewis et al.</author>


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<title>Consumption of Omega-3 Fatty Acid-Enriched Eggs and Serum Lipids in Humans</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/2</link>
<guid isPermaLink="true">http://digitalcommons.unl.edu/nutritionfacpub/2</guid>
<pubDate>Mon, 13 Jun 2011 13:35:39 PDT</pubDate>
<description>
	<![CDATA[
	<p>This study examined the effectiveness of consuming omega-3 fatty acid-enriched eggs (Omega Eggs) in increasing total diclary omega-3 fatty acids. Also examined was the impact of Omega Egg consumption on serum lipids. Sixteen hypercholesterolemic men and women with baseline serum total cholesterol concentrations of 5.17-7.76 mmol/L (200-300 mg/dL) followed the National Cholesterol Education Program Step I diet guidelines under the following conditions: (a) Step 1 diet without eggs, (b) Step I diet plus 12 regular eggs per week, and (c) Step I diet plus 12 Omega Eggs per week. The study design was a repeated 3 x 3 Latin square so that each subject received each of the three diet treatments. Consumption of Omega Eggs significantly increased omega-3 fatty acid intake (1.18 g/day) compared to consumption of regular eggs (0.71 g/day) or no eggs (0.81 g/day). The Omega Egg treatment did not significantly alter serum cholesterol or triacylglycerol concentration when all 16 subjects were included in the analysis. However, three subjects showed a significant increase in serum total cholesterol concentration when consuming regular eggs relative to no eggs. When these "responders" consumed Omega Eggs, serum tolal cholesterol concentration did not increase, despite a 3-fold increase in cholesterol intake relative to no egg treatment. These data suggest that Omega Eggs (12/week) can be included in the National Cholesterol Education Program Step I diet without increased serum total cholesterol or triacylglycerol concentration. In this way, the nutritional benefits of eggs could be realized without the detrimental effects of increased cholesterol intake.</p>

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<author>Ji-Young Lee et al.</author>


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<title>Lysine residues in N-terminal and C-terminal regions of human histone H2A are targets for biotinylation by biotinidase</title>
<link>http://digitalcommons.unl.edu/nutritionfacpub/1</link>
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<pubDate>Wed, 25 Apr 2007 08:01:17 PDT</pubDate>
<description>
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	<p>In eukaryotic cell nuclei, DNA associates with the core histones H2A, H2B, H3 and H4 to form nucleosomal core particles. DNA binding to histones is regulated by posttranslational modifications of N-terminal tails (e.g., acetylation and methylation of histones). These modifications play important roles in the epigenetic control of chromatin structure. Recently, evidence that biotinidase and holocarboxylase synthetase (HCS) catalyze the covalent binding of biotin to histones has been provided. The primary aim of this study was to identify biotinylation sites in histone H2A and its variant H2AX. Secondary aims were to determine whether acetylation and methylation of histone H2A affect subsequent biotinylation and whether biotinidase and HCS localize to the nucleus in human cells. Biotinylation sites were identified using synthetic peptides as substrates for biotinidase. These studies provided evidence that K9 and K13 in the N-terminus of human histones H2A and H2AX are targets for biotinylation and that K125, K127 and K129 in the C-terminus of histone H2A are targets for biotinylation. Biotinylation of lysine residues was decreased by acetylation of adjacent lysines but was increased by dimethylation of adjacent arginines. The existence of biotinylated histone H2A in vivo was confirmed by using modification-specific antibodies. Antibodies to biotinidase and HCS localized primarily to the nuclear compartment, consistent with a role for these enzymes in regulating chromatin structure. Collectively, these studies have identified five novel biotinylation sites in human histones; histone H2A is unique among histones in that its biotinylation sites include amino acid residues from the C-terminus.</p>

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<author>Yap Ching Chew et al.</author>


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