The Mitochondrial Genome of Eleusine indica and Characterization of Gene Content within Poaceae

Authors

Nathan D. Hall, Auburn University
Hui Zhang, Auburn University
Jeffrey P. Mower, University of Nebraska-LincolnFollow
Joseph Scott McElroy, Auburn University
Leslie R. Goertzen, Auburn UniversityFollow

ORCID IDs

Nathan D. Hall

Document Type

Article

Date of this Version

2019

Citation

Genome Biol. Evol. 12(1):3684–3697. doi:10.1093/gbe/evz229

Comments

(c) The Author(s) 2020.

Abstract

Plant mitochondrial (mt) genome assembly provides baseline data on size, structure, and gene content, but resolving the sequence of these large and complex organelle genomes remains challenging due to fragmentation, frequent recombination, and transfers of DNA from neighboring plastids. Themt genome for Eleusine indica (Poaceae: goosegrass) is comprehensibly analyzed here, providing key reference data for an economically significant invasive species that is also the maternal parent of the allotetraploid crop Finger millet (Eleusine coracana). The assembled E. indica genome contains 33 protein coding genes, 6 rRNA subunits, 24 tRNA, 8 large repetitive regions 15 kb of transposable elements across a total of 520,691 bp. Evidence of RNA editing and loss of rpl2, rpl5, rps14, rps11, sdh4,and sdh3 genes is evaluated in the context of an updated survey of mt genomic gene content across the grasses through an analysis of publicly available data. Hypothesized patterns of Poaceae mt gene loss are examined in a phylogenetic context to clarify timing, showincl2 was transferred to the nucleus from the mitochondrion prior to the origin of the PACMAD clade.