Agronomy and Horticulture, Department of
Document Type
Article
Date of this Version
9-20-2024
Citation
Plant Direct. 2024;8:e70010. https://doi.org/10.1002/pld3.70010
Abstract
Sunflower (Helianthus annuus L.) plays an essential role in meeting the demand for edible oil worldwide. The yield of sunflower seeds encompasses several component traits, including the disc diameter. Over three consecutive years, 2019, 2020, and 2022, we assessed phenotypic variation in disc diameter across a diverse set of sunflower accessions (N = 342) in replicated field trials. Upon aggregating the phenotypic data from multiple years, we estimated the broad sense heritability (H2) of the disc diameter trait to be 0.88. A subset of N = 274 accessions was genotyped by using the tunable genotyping-by-sequencing (tGBS) method, resulting in 226,779 high-quality SNPs. Using these SNPs and the disc diameter phenotype, we conducted a genome-wide association study (GWAS) employing two statistical approaches: the mixed linear model (MLM) and the fixed and random model circulating probability unification (farmCPU). The MLM and farmCPU GWAS approaches identified 106 and 8 significant SNPs located close to 53 and 21 genes, respectively. The MLM analysis identified two significant peaks: a prominent signal on chromosome 10 and a relatively weaker signal on chromosome 16, both of which were also detected by farmCPU. The genetic loci associated with disc diameter, as well as the related candidate genes, present promising avenues for further functional validation and serve as a basis for sunflower oil yield improvement.
Xu PD 2024 Dissecting the genetic SUPPL2.pdf (2183 kB)
Xu PD 2024 Dissecting the genetic SUPPL3.xlsx (22 kB)
Xu PD 2024 Dissecting the genetic SUPPL4.xlsx (19 kB)
Xu PD 2024 Dissecting the genetic SUPPL5.xlsx (10 kB)
Xu PD 2024 Dissecting the genetic SUPPL6.xlsx (12 kB)
Xu PD 2024 Dissecting the genetic SUPPL7.xlsx (16 kB)
Xu PD 2024 Dissecting the genetic SUPPL8.xlsx (28 kB)
Xu PD 2024 Dissecting the genetic SUPPL9.pdf (152 kB)
Included in
Agricultural Science Commons, Agriculture Commons, Agronomy and Crop Sciences Commons, Botany Commons, Horticulture Commons, Other Plant Sciences Commons, Plant Biology Commons
Comments
Open access.