Agronomy and Horticulture Department


Date of this Version



Published in Crop Sci. 47:943–950 (2007).


U.S. Government Work


Fertility restoration of sorghum [Sorghum bicolor (L.) Moench] lines carrying the IS1112C (A3 group) male-sterile cytoplasm has been documented as a two-gene gametophytic mechanism involving complementary action of restoring alleles designated Rf3 and Rf4, as derived from IS1112C. Fertility restoration capability has also been reported from sudangrass (S. bicolor subsp. drummondii) populations. We describe characteristics of a fertility restoration system derived from sudangrass, in which male-sterile individuals were observed at high frequency in backcross and F2-F3 segregating populations. Segregation analyses were consistent with a sporophytic restoration system involving two complementary genes. Pollen iodine staining in fertile progeny indicated that the restorers were not efficacious, and fertility was decreased in progeny of backcrosses. Silencing of restoring alleles through paramutation might be operative in these examples. Sudangrass-derived fertility restoration did not involve enhanced transcript processing of the chimeric mitochondrial open reading frame orf107. Thus male sterility induced by the A3 cytoplasm can be restored through different mechanisms.