Animal Science, Department of

 

First Advisor

Gary A. Sullivan

Second Advisor

Jordan C. Wicks

Committee Members

Mary-Grace Danao

Date of this Version

12-2024

Document Type

Thesis

Citation

A thesis presented to the faculty of the Graduate College at the University of Nebraska in partial fulfillment of requirements for the degree of Master of Science

Major: Animal Science

Under the supervision of Professors Gary A. Sullivan and Jordan C. Wicks

Lincoln, Nebraska, December 2024

Comments

Copyright 2024, Ashley A. Hahn. Used by permission

Abstract

Previous studies have shown high-pressure processing (HPP) can be utilized to improve lean color of dark-cutting beef color by increasing the lightness and redness of steaks. However, the optimal parameters of length of time product is held under pressure and at which pressure must be applied have yet to be fully defined. Furthermore, the impact of HPP on color labile muscles in dark-cutting beef are currently unknown. Therefore, two studies were designed and specific efforts were focused on first defining optimal parameters for color stable muscles, followed by determining the effect of HPP on dark-cutting color labile muscles.

In the first study, pressures of 300 and 450 MPa were shown to be effective at increasing the lightness and redness of dark-cutting steaks regardless of holding time. A pressure of 240 MPa was also implemented but showed limited effects. On the other hand, steaks subjected to the 450 MPa pressure presented a pale and negative visual appearance, the greatest surface discoloration, and lipid oxidation for the higher holding times (30, 60, and 90 s; P < 0.05).

In the second study which examined HPP impact on dark-cutting color labile muscle (Psoas Major), pressures of 300 and 450 MPa resulted in increased lightness (L*) and yellowness (b*) but decreased redness (a*) in treated steaks. The 450 MPa treatment group had the most impact with the greatest lightness, lowest redness, and increased lipid oxidation. Furthermore, HPP treatment resulted in greater surface discoloration, higher color scores and greater paleness (P < 0.05).

Advisors: Gary A. Sullivan and Jordan C. Wicks

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