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Thesis (M.S.)—University of Nebraska—Lincoln, 1969. Department of Biochemistry and Nutrition.


Copyright 1969, the author. Used by permission.


In hope of understanding the nature of obligate parasitism, considerable effort has been spent in study of the biochemical events in germination rust uredospores. In recent years, studies have indicated that there is no net RNA or protein synthesis during germination. These findings have led to the hypothesis that a deficiency in either protein or RNA synthesis may be a factor which requires these organisms to use a living host tissue for growth and development.

The present research was undertaken to re-examine these questions of nucleic acid and protein synthesis during the early stages of bean rust uredospore (Uromyces phaseoli) germination. Rather than determining nucleic acids and proteins after extraction by conventional procedures, crystalline enzymes were used to hydrolyze these macromolecules that are present in uniformly 14C-labeled uredospores. The desired products of enzymatic hydrolysis were purified by chromatographic techniques, and then qualitatively determined by radioactivity assays.

Advisor: J. M. Daly