Libraries at University of Nebraska-Lincoln


Document Type


Date of this Version



Thesis (M.S.)—University of Nebraska—Lincoln, 1970. Department of Veterinary Science.


Copyright 1971, the author. Used by permission.


Pseudorabies (Aujeszky’s Disease, Mad Itch, Infectious bulbar paralysis) is an infectious disease of cattle, swine, sheep, rates, dogs, and cats caused by a Herpes virus and characterized by intense, localized irritation of the skin, convulsions, prostration, and death. The disease in cattle is characterized by a marked local pruritus and a rapidly fatal ascending encephalomyelitis. Bergman and Becker (1967) inoculated swine and rabbits with Herpesvirus suis by intramuscular and intranasal-oral routes. They suggested a neural spread to the brain when animals were inoculated by these routes.

The following experiments were undertaken in an attempt to confirm these findings and compare the pathogenesis of H. suis based on different routes of inoculation. Light microscopy, immunofluorescence, viral isolation, and electron microscopy methods were used to locate the virus in the animal body. It is hoped this study will help to clarify the basic question of neural or hematogenous spread of the virus.

Advisor: E. L. Stair, Jr.