Date of this Version
Thesis (M.S.)—University of Nebraska—Lincoln, 1971. Department of Chemistry.
The interaction of specific halogenated methylated ketones derivatives with proteins is a powerful technique for studying the interaction of substrate analogs with proteolytic enzymes e.g. chymotrypin and trypsin. Since the mechanism of action and the functional groups involved in the mechanism are unknown in the amnio acyl tRNA synthetases, the use of chloroketone analogs of amino acids may serve as the powerful tool to investigate the nature of this reaction. Both the activation of amino acid and the subsequent transfer to tRNA, these studies were undertaken to ascertain to what extent these chloroketones may be useful for these purposes.
Advisor: Robert B. Johnston