Use of thiolated oligonucleotides as anti-fouling diluents in electrochemical peptide-based sensors

Authors

Adam McQuistan, University of Nebraska-Lincoln
Anita J. Zaitouna, University of Nebraska-Lincoln
Elena Echeverria, University of Nebraska-Lincoln
Rebecca Y. Lai, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2014

Citation

Chem. Commun., 2014, 50, 4690--4692; DOI: 10.1039/c4cc01290a.

Comments

Copyright 2014 The Royal Society of Chemistry

Abstract

We incorporated short thiolated oligonucleotides as passivating diluents in the fabrication of electrochemical peptide-based (E-PB) sensors, with the goal of creating a negatively charged layer capable of resisting nonspecific adsorption of matrix contaminants. The E-PB HIV sensors fabricated using these diluents were found to be more specific and selective, while retaining attributes similar to the sensor fabricated without these diluents. Overall, these results highlight the advantages of using oligonucleotides as anti-fouling diluents in self-assembled monolayer-based sensors.