Published Research - Department of Chemistry


Date of this Version



Published in final edited form as: Anal Biochem. 2008 May 1; 376(1): 154–156. Version presented here is from NIH PubMed Central


Copyright Elsevier Inc. Used by permission


A new method for the immobilization of α1-acid glycoprotein (AGP) in HPLC columns was recently described for applications such as drug binding studies. Part of this earlier work used self-competition zonal elution studies to measure association equilibrium constants between immobilized AGP and R- or S-propranolol. It was later found that analysis of these data by a common equation derived for linear elution conditions gave erroneous values for experiments actually conducted under nonlinear conditions. This report discusses the nature of this error and uses frontal analysis to estimate the true binding strength between R- and S-propranolol and HPLC columns containing immobilized AGP.