Animal Science Department

 

Date of this Version

2009

Comments

Published in 2009 Nebraska Swine Report. Prepared by the staff in Animal Science and cooperating Departments for use in Extension, Teaching, and Research programs. Copyright © 2009, The Board of Regents of the University of Nebraska on behalf of the University of Nebraska-Lincoln Extension.

Abstract

Preliminary experiments were carried out to evaluate in vivo expression of zinc transporter (ZnT) 1 mRNA in a panel of tissues obtained from weaned pigs and to evaluate in vitro changes in Z n T l mRNA in a porcine jejunal epithelial cell line (IPEC-J2) treated with low (40 yM) or high (80 yM) concentrations of zinc sulphate (ZnSO,) or, in a separate experiment, with low (50 yg/ml) or high (1 00 yg/mL) concentrations of antibiotics (as gentamicin; GENT). Lipopolysaccharide (LPS; 10 ng/mL) was included as a negative control in both experiments. Zinc transporter 1 mRNA was detected in all tissues evaluated with the greatest level of expression observed in the tonsil. A treatment X time interaction was not observed for IPEC-J2 cells treated with ZnSO,; however, the addition of high ZnSO, tended (P = 0.08) to increase Z n T l mRNA expression when means were averaged among all time points. Exposure of IPEC-J2 cells to GENT resulted in a significant treatment x time interaction (P < 0.005) with increases in Z n T l mRNA observed at 3 (low GENT) and 6 (low and high GENT) hours post treatment compared to CTL- or LPSt eated cells. This research indicates that Z n T l mRNA is differentially expressed in vivo, in a panel of porcine tissues, and in vitro, in IPEC-J2 cells exposed to low or high concentrations of zinc or antibiotics.

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