Off-campus UNL users: To download campus access dissertations, please use the following link to log into our proxy server with your NU ID and password. When you are done browsing please remember to return to this page and log out.

Non-UNL users: Please talk to your librarian about requesting this dissertation through interlibrary loan.

Generation and Evaluation of Modified Opaque-2 (o2) Popcorn Suggests a Route to Quality Protein Popcorn (QPP)

Ying Ren, University of Nebraska - Lincoln


I have been working on a Quality Protein Popcorn breeding project where QPM conversion is carried out simultaneously for several elite popcorn germplasms. During my study in the graduate program, I led the following aspects of the Quality Protein Popcorn Breeding Project: 1. Identified suitable QPMs as opaque-2 allele donors. 2. Examined the feasibility of quick introgression of the opaque-2 allele into popcorn lines via marker-assisted selection. 3. Monitored modification by SDS-PAGE zein profiling and light box phenotypic selection to make sure multiple modifier loci for opaque-2 were incorporated each time generation advancement was carried out. 4. Carried out high throughput DNA extraction using the BioSprint Workstation and performed genotyping to guide field selection. 5. Principle Component Analysis (PCA) of amino acid profiles from UPLC (Ultra Performance Liquid Chromatography) of candidate QPP lines along with corresponding parental lines (QPM and popcorn). (in collaboration with Dr. Ruthie Angelovici from University of Missouri Columbia, MO)

Subject Area


Recommended Citation

Ren, Ying, "Generation and Evaluation of Modified Opaque-2 (o2) Popcorn Suggests a Route to Quality Protein Popcorn (QPP)" (2018). ETD collection for University of Nebraska-Lincoln. AAI13418977.