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Development and utilization of genomic resources for genetic improvement of proso millet (Panicum miliaceum L.)
Proso millet (2n=4x=36), an allotetraploid self-pollinated cereal with highest water-use efficiency, is the best rotational crop in dryland production system in the semi-arid High Plains of the USA and elsewhere in the world. Breeding and genomic research in this crop is very limited. Overall goal of this dissertation is to develop proso millet genetic and genomic resources (germplasm characterization, molecular marker development, and QTL mapping) for the genetic improvement through conventional and molecular breeding. For marker development, a total of 348 SSRs were developed for proso millet using cross-species genomic resources. In genetic diversity, 90 diverse genotypes, considered as proso millet core collection, were clustered into three groups based on 1287 SSR marker alleles, amplified by the 100 SSR markers. Genotypes were clustered corresponding to their geographic origin, pedigree and morpho-agronomic traits with few exceptions. In germplasm characterization, seventy-seven of the 90 genotypes showed a wide range of variation for nine morpho-agronomic traits and formed six clusters based on the phenotypic data. Genotypes were significantly different for all the traits across locations and many traits were highly correlated. Genotypes with resistance to lodging and grain shattering, two very important traits in proso millet breeding, were identified. The fourth objective, the first genetic linkage map of proso millet was developed using 833 GBS-SNP markers in 93 RILs and the map consisted 18 major and 84 minor linkage groups. The 18 major linkage groups spanned 2137 cM of proso millet genome with an average density of 18 cM. A total of 17 QTLs (LOD = 2.55 to 3.98) for seven morpho-agronomic traits were detected, which explained 13.2 to 34.7% phenotypic variance. DNA markers flanking the QTLs were identified, which could be useful in marker-assisted selection of these traits. The results of these studies will have significant utilization in proso millet breeding, genetics, and genomics research.
Rajput, Santosh Ghashiram, "Development and utilization of genomic resources for genetic improvement of proso millet (Panicum miliaceum L.)" (2015). ETD collection for University of Nebraska - Lincoln. AAI3716450.