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RAPD molecular markers for the genes controlling seedling lethality and leaf crippling in common beans

Martha B Davila Ponce, University of Nebraska - Lincoln

Abstract

The genes involved in F$\sb1$ seedling abnormal development and lethality in inter-gene pool crosses of Phaseolus vulgaris L. were designated previously as $Dl\sb1$ (MesoAmerican: MA), and $Dl\sb2$ (Andean:A). The different degrees of leaf crippling (C) in segregating populations of crosses was reported previously as due to the interaction between the $Dl\sb1$ or $Dl\sb2$ loci, growing environment, and the lcr allele. The objective here was to identify RAPD markers for the genes for crippling (Lcr) and seedling lethality (Dl) using the bulked segregant analysis procedure for segregating populations of MA gene pool. Crosses were made between C lines, FB 10413-24-2 $(Dl\sb1Dl\sb1dl\sb2dl\sb2lcrlcr),$ and WA 7807-305 $(Dl\sb1Dl\sb1dl\sb2dl\sb2lcrlcr),$ and normal (N) parents 'Carioca' $(Dl\sb1Dl\sb1dl\sb2dl\sb2LcrLcr)$ and 'Brasil 2' $(dl\sb1dl\sb1dl\sb2dl\sb2lcrlcr)$ (postulated genotypes in parenthesis). The F$\sb2$ from crosses FB 10413-24-2 x 'Carioca' and FB 10413-24-2 x 'Brasil 2', respectively, segregated 13N:3C. An hypothesis of two interacting genes with Lcr acting as an inhibitor was confirmed in the F$\sb3.$ The new genotype $dl\sb1dl\sb1dl\sb2dl\sb2LcrLcr$ for 'Carioca' and 'Brasil 2' was now proposed. F$\sb3$ families segregating 3C:1N were used to detect a RAPD molecular marker for the $Dl\sb1$ gene. RAPD marker OPQ3 $\sb{500}$ was linked in coupling phase to the $Dl\sb1$ locus at 18.8 cM. F$\sb3$ families segregating 3N:1C were used to detect a RAPD molecular marker for the Lcr gene. RAPD marker OPB10 $\sb{500}$ was linked in coupling phase to the Lcr locus at 31.7 cM. The F$\sb1$ of the cross between the two C lines FB10413-24-2 x WA 7807-305 was normal F$\sb1$ indicating that two different genes were involved for the cripple trait. A new gene for the cripple trait was designated as Lcr-2. Lcr-2, the cripple gene present in WA 7807-305, was tagged with RAPD markerOPB10 $\sb{600}$ and was linked in coupling phase at 19.9 cM. In the cross FB 10413-24-2 x 'Brasil 2' duplicate recessive genes $(dpy\sb1$, and $dpy\sb2)$ were found to control the dwarf pale yellow mutant (DPY). No association was found between C, N and DPY.

Subject Area

Molecular biology|Plant propagation|Botany|Agronomy

Recommended Citation

Davila Ponce, Martha B, "RAPD molecular markers for the genes controlling seedling lethality and leaf crippling in common beans" (1997). ETD collection for University of Nebraska-Lincoln. AAI9812351.
https://digitalcommons.unl.edu/dissertations/AAI9812351

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