U.S. Department of Agriculture: Animal and Plant Health Inspection Service


Date of this Version

March 2004


Published in JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Vol. 27, No. 5, pp. 897–909, 2004.


Acetaminophen was extracted from brown treesnakes (Boiga irregularis) and analyzed by reversed-phase high-performance liquid chromatography (HPLC). Acetaminophen was quantified by UV absorbance at 250 nm. Recoveries were determined by analyzing acetaminophen-fortified blank homogenized tissue. The mean recovery of acetaminophen in whole body brown treesnakes was 87.9% + 5.9% and 92.2% + 5.8% for the fortification levels of 20 and 2400mg/g, respectively. The method’s limit of detection (MLOD) with UV detection was 0.70mg/g.