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The study examined the competency requirements and levels of library and information professionals (LIPs)for updating information services for agricultural research in Nigerian university libraries. Employing descriptive survey design, all the 89 library and information professionals (LIPs) in six federal university libraries (three conventional and three agricultural universities) selected based on purposive sampling method were used for the study. The research employed the use of structured questionnaire, code-named, 'UPINSEQ' and an observation checklist as instrument for collecting data. Data collected were analyzed using frequency counts, percentages, mean and standard deviation while the null hypotheses were tested at 0.05 level of significance using the chi-square. Findings revealed that among the competencies required for updating information services for agricultural research include skills in ICT use in information services; skills in conventional library information services, wide knowledge of sources of agricultural information; wide general subject expertise in agricultural science, and communication skills. It is recommended that ICT facilities should be made readily available for training and for accessing information for agricultural research in the university libraries.