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The study aims to investigate the level of awareness and use of Web 2.0 tools by Library and Information Science (LIS) students at the University of Nigeria, Nsukka. The study adopted a descriptive survey method and questionnaire was used to collect data from 220 respondents. The results show that the LIS students of UNN are quite familiar with some Web 2.0 tools such as Social networking sites, Instant Messaging, blogs and Wikis, while, they are not familiar with tools such as RSS feeds, Podcasts, and social bookmarks. The study revealed that the most frequently used Web 2.0 tools are Facebook, followed by YouTube and Wikis. The UNN LIS students indicated communicating with friends/family, exchanging opinions/news for their personal life, and acquaintances/meet people as the top reasons for joining social networks. The study also revealed that UNN students acquired the skills to use Web 2.0 tools through friends, followed by through self-practice, While only a few students indicated learning the skills through the library school. It is recommended that LIS schools incorporate units about Web 2.0 tools into their curricula to prepare future information professionals by developing their skills for future challenges of using such tools.