This paper investigated the relationship among preservation knowledge, perception and attitude of library personnel towards preservation of information resources in Nigerian federal university libraries. Simple random sampling technique was used to select twenty university libraries from the twenty four federal university libraries in Nigeria. All the 660 library personnel in the selected libraries constituted the study sample. Data was collected by a questionnaire administered on the 660 personnel out of which 510 copies were returned. The response rate was 77.3%. The study found that knowledge and perception of library personnel about preservation of information resources correlate significantly with their attitude towards preservation. The study also established that a significant relationship exists between age of library personnel and their perception of preservation. The study concludes that knowledge and perception of preservation of information resources jointly influence attitude towards preservation of information resources. It is therefore recommended that the library management should improve the attitude of the personnel through training and development.