A study of the information seeking behaviour of students is an important pre-requisite for a library to provide efficient information resources and services to meet their information needs. The aim of the study is to investigate the information needs, information sources, information seeking behaviours, library use, and challenges to information seeking behaviour of graduate students of the University of Professional Studies, Accra (UPSA). The study employed the survey methodology to select 121 graduate students using stratified random sampling. The main data collection instrument was the questionnaire. The main information needs of students identified in the study include career information, self-development, employment, course information, health, entertainment, sports, and religion. They use five main categories of information sources: interpersonal, internet, mass media, electronic and print. Their information seeking behaviours include active search, passive search, and ongoing search. The challenges they encountered in seeking information are mostly technical, which are, unstable internet connection, low internet speed, issues regarding accessibility using password, and inadequate computers. The study concluded that it is important for the university authorities to work together with the library to address these problems so that the information needs of students are fully met to enhance their academic work.