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The aim of study is to examine the impact of knowledge sharing tools (i.e., technology and organization rewards) on knowledge transfer in the Higher Education Institutions (HEIS) of Sindh, Pakistan. The study utilized quantitative method. The population of the study was the employees who are working in HEIs of Sindh Province. The cluster random sampling technique was implemented to collect primary data from 750 respondents. The survey method was used in questionnaire to collect the data from employees of HEIs. The results of the research study indicate that knowledge sharing tools and organization rewards and technology have a significant impact on knowledge transfer. The results also confirm that motivation mediates the relationship among organization reward, technology, and knowledge transfer. Furthermore, the organization reward and technology along with motivation endorse the knowledge transfer to individuals and organizations. This study has sought the contribution in managerial and theoretical perspectives considering technology and organization reward as knowledge sharing tool effect on knowledge transfer with mediation analysis of motivation which improves the organization’s effectiveness. The comparative study may be conducted in the future or in another region of the country.