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This paper investigated the influence of ICT skills on the research output of academic librarians in public universities in the South-West, Nigeria. The study was guided by three objectives and three corresponding research questions. A survey research design method was adopted for the study. The total population for the study was two hundred and twenty (220) librarians in the South-West Nigerian universities. Hence, the total population was adopted. A questionnaire was the main instrument used for data collection. Two hundred and three (203) copies of the questionnaire were returned and used for the analysis. Data were analysed using descriptive (frequency count, tables, percentages, mean, and standard deviation) and inferential (simple linear regression) statistics. The results of the analysis revealed that academic librarians’ level of research output was moderate in quality and quantity. The result also showed that the level of ICT skills among academic librarians in public universities in South-West Nigeria was high. ICT skills have a positive and significant influence on research output among academic librarians in public universities in South-West, Nigeria. Based on the findings, it was recommended that academic librarians should endeavour to publish more textbooks, journal articles, conference proceedings, monographs, book chapters, and technical reports, in high rated journals for more visibility.