Agronomy and Horticulture Department

 

Backcross Breeding 2: The Backcrossing Process

Date of this Version

2003

Document Type

Article

Citation

Plant and Soil Sciences eLibrary Lesson.

Comments

Copyright 2003, the authors. Used by permission.

Peer-reviewed web lesson, JNRLSE approved, 2003.

This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, adminstered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA or NSF.

Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC). Any opinions, findings, conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture.

Abstract

This lesson discusses the final stage of developing genetically engineered crops. The need for backcrossing, and the steps of this breeding method are described. Yield lag, yield drag, and gene stacking are also discussed.

Overview and Objectives - The Backcrossing Process

This lesson discusses the final stage of developing genetically engineered crops. The need for backcrossing, and the steps of this breeding method are described. Yield lag, yield drag, and gene stacking are also discussed.

At the completion of this lesson you should be able to:

  • Explain why backcross breeding is necessary in developing genetically engineered lines.
  • Describe how backcross breeding is done.
  • Explain the advantages and disadvantages of using selectable markers, ELISA, and PCR in screening for transgenic plants.
  • Predict the amount of time it would take for a specific backcrossing program to be completed.
  • Distinguish between the concepts of yield lag and yield drag and describe the potential causes of each.
  • Define 'gene stacking' and list the two possible ways to stack genes.

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