Agronomy and Horticulture Department

 

Title

Gene Cloning

Date of this Version

2019

Document Type

Article

Citation

Plant and Soil Sciences eLibrary (PASSeL) Lesson

Comments

Copyright © 2019 Patricia Hain and Don Lee. Used by permission.

Peer reviewed web lesson JNRLSE approved 2003

This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, administered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA or NSF.

Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept. of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC). Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.

Abstract

Overview and Objectives - Gene Cloning

This lesson covers the utilization of gene cloning to isolate and copy a specific gene of interest. The transformation of bacteria with plasmids containing antibiotic resistance genes to make gene libraries and the selection of bacteria colonies that contain the specific gene of interest are described.

At the completion of this lesson, you should be able to:

  • Define gene cloning and describe its role in genetic engineering.
  • Illustrate the basic steps in gene cloning.
  • Explain why gene libraries need to be made in order to clone a gene of interest.
  • Explain why antibiotic resistance genes are sometimes present in transgenic plants.
  • Identify the final product of gene cloning.

Modules:

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