Gene Cloning Part 2: Making and Screening Gene Libraries
Date of this Version
Plant and Soil Sciences eLibrary (PASSeL) Lesson
This lesson teaches how a specific gene can be identified from among the thousands of genes that can be cloned from an organism.
Objectives for Gene Cloning Part 2
At the conclusion of this lesson you should be able to:
- Describe the library screening process including why it is important in gene cloning.
- Determine which screening method would be appropriate for a given gene library.
- Compare traditional gene cloning with modern methods of automated DNA sequencing and database analysis techniques for gene cloning.
- Lesson home
- Objectives for Gene Cloning Part 2
- Final Step: Screening Gene Libraries
- How do the colonies in the library differ?
- In situ Detection Methods; DNA homology
- In situ Detection of Proteins
- Detection of Proteins in cDNA Libraries
- Detection of Proteins in a Genomic Library
- Cloning from a Gene Database: Bioinformatics
- A summary of library screening strategies