MB2 - Selection of Markers for Molecular Breeding
Date of this Version
Plant and Soil Sciences eLibrary (PASSeL) Lesson
Molecular Markers (variants of DNA sequences) are identifiers (or “tags”) of certain aspects of a phenotype and/or genotype which could be exploited by plant breeders for incorporating desirable traits into their advancing germplasm. It is important therefore that we understand the proper usage of markers, be able to select the proper marker types, and have an understanding of DNA extraction protocols and the general organization of the crop genome of interest. This lesson is written for plant breeders who are interested in or beginning to use molecular markers.
At the end of this lesson you will be able to:
- Categorize marker types, describe the need for using molecular markers in plant breeding programs, and list the desirable qualities of markers.
- List the common steps in DNA extraction protocols required to produce sufficient quality and quantity of DNA.
- Know the molecular or genetic aspects of markers to ensure their proper usage.
- Gain understanding of the DNA structure, genome organization, and the role and design of primers in the PCR process.