Plant Pathology Department


Date of this Version



Published in Botanical Gazette, Vol. 113, No. 4 (Jun., 1952), pp. 482-484 Copyright 1952 University of Chicago Press. Used by permission.


This report deals with the effect of certain synthetic plant growth-regulators on the activity of alpha-amylase (1) secreted by virus tumor tissue grown in vitro. Previous studies have lacked adequately controlled conditions and have been carried out with enzyme preparations obtained through the usual methods of destruction of cells. In this particular study (a) the assay employed to measure enzyme activity includes control of pH and temperature and (b) this enzyme, being secreted, is not subject to the drastic preparative procedures involving destruction of cellular structure usually necessary to obtain enzymes.