Wheat Streak Mosaic Virus Cylindrical Inclusion Body Protein

Authors

M. K. Brakke, University of Nebraska-Lincoln
E. M. Ball, University of Nebraska-Lincoln
Y. H. Hsu, National Chung Hsing University
W. G. Langenberg, University of Nebraska-Lincoln

Date of this Version

1987

Comments

Published in J. gen. Virol. (1987), 68, 281-287.

Abstract

A protein of apparent molecular weight 66000 was purified from wheat plants infected with wheat streak mosaic virus. Antiserum to this protein, labelled with gold, specifically stained cylindrical inclusions in ultrathin sections of virus-infected cells. Antiserum to the M_r 66000 protein did not react with capsid protein in Western blots, nor did antiserum to capsid protein react with the M_r 66000 protein. Both antiserum reacted with homologous antigens. The concentration of the M_r 66000 protein in extracts from infected leaves was about 100 µg per g of leaves, which is higher than the usual concentration of virions.