Date of this Version
UCARE Poster session, University of Nebraska-Lincoln Research Fair, April 2016, Lincoln, NE.
This experiment consisted of the controlled differentiation of H9 embryonic stem cells to mesenchymal stem cells. Though this experiment was repeated twice and improvement was seen in these repetitions, the cells were only able to be partially differentiated. However, the morphology of the differentiated cells is similar to those of healthy adult mesenchymal stem cells.
The progression of the differentiation can be seen in the microscope slide photos below. Throughout the differentiation, there occurred a decrease in cell survival and reduction of cell growth, but an increase in mesenchymal stem cell morphology. Throughout the last week of the differentiation, very little cell growth was seen and the cells were fixed on Day 20. With this, the cells were unable to sustain survival and growth to complete the four passages required for complete mesenchymal stem cell differentiation.