UCARE: Undergraduate Creative Activities & Research Experiences

 

Date of this Version

Spring 4-12-2020

Document Type

Poster

Citation

Poster presentation, UCARE Research Symposium, University of Nebraska-Lincoln, Spring 2020.

Comments

Copyright 2020 by the authors.

Abstract

This experiment looked into the effects of dietary microbiota accessible carbohydrates (MACs)on the composition of human GI microbe colonies and their susceptibility to pathogen infection. Three different concentrations of MAC ingrained media were tested in mini bioreactor arrays, treated with antibiotics, infected with Clostridioides difficile, then allowed to recover. Microbe community was examined for strain composition and diversity before and after the antibiotic treatment and Clostridioides difficile infection. After the initial mini bioreactor run, individual isolates were investigated in their ability to respond to specific MACs Arabinoglactina, Inulin, and Soluble starch at experimental concentrations.

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