U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska


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Li L, Bannantine JP, Campo JJ, Randall A, Grohn YT, Katani R, et al. (2017) Identification of sero-reactive antigens for the early diagnosis of Johne’s disease in cattle. PLoS ONE 12(9): e0184373. https://doi.org/10.1371/journal. pone.0184373


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Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease (JD), a chronic intestinal inflammatory disease of cattle and other ruminants. JD has a high herd prevalence and causes serious animal health problems and significant economic loss in domesticated ruminants throughout the world. Since serological detection of MAP infected animals during the early stages of infection remains challenging due to the low sensitivity of extant assays, we screened 180 well-characterized serum samples using a whole proteome microarray from Mycobacterium tuberculosis (MTB), a close relative of MAP. Based on extensive testing of serum and milk samples, fecal culture and qPCR for direct detection of MAP, the samples were previously assigned to one of 4 groups: negative low exposure (n = 30, NL); negative high exposure (n = 30, NH); fecal positive, ELISA negative (n = 60, F+E-); and fecal positive, ELISA positive (n = 60, F+E+). Of the 740 reactive pro- teins, several antigens were serologically recognized early but not late in infection, suggest- ing a complex and dynamic evolution of the MAP humoral immune response during disease progression. Ordinal logistic regression models identified a subset of 47 candidate proteins with significantly different normalized intensity values (p

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