Date of this Version
J Microbiol. 2003 ; 41(4): 295–299.
Adenovirus (Ad) precursor to the terminal protein (pTP) plays an essential roles in the viral DNA
replication. Ad pTP serves as a primer for the synthesis of a new DNA strand during the initiation
step of replication. In addition, Ad pTP forms organized spherical replication foci on the nuclear
matrix (NM) and anchors the viral genome to the NM. Here we identified the interferon inducible
gene product 1-8D (Inid) as a pTP binding protein by using a two-hybrid screen of a HeLa cDNA
library. Of the clones obtained in this assay, nine were identical to the Inid, a 13-kDa polypeptide
that shares homology with genes 1-8U and Leu-13/9-27, most of which have little known functions.
The entire open reading frame (ORF) of Inid was cloned into the tetracycline inducible expression
vector in order to determine the biological functions related with adenoviral infection. When Inid
was introduced to the cells along with adenoviruses, fifty to sixty percent of Ad-infected cells
expressing Inid had rounded morphology, which was suggestive of apoptosis. Results from the
terminal deoxynucleotidyl transferase (TdT) and DNA fragmentation assays confirmed that Inid
induces apoptosis in Ad-infected or in uninfected cells. The Inid binding to pTP may target the cell
for apoptotic destruction as a host defense mechanism against the viral infection.
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