Reversing resistance to tembotrione in an Amaranthus tuberculatus (var. rudis) population from Nebraska, USA with cytochrome P450 inhibitors

Authors

Maxwel C. Oliveira, University of Nebraska-LincolnFollow
Todd A. Gaines, Colorado State University in Fort CollinsFollow
Franck E. Dayan, Colorado State University in Fort CollinsFollow
Eric L. Patterson, Colorado State University in Fort CollinsFollow
Amit J. Jhala, University of Nebraska-LincolnFollow
Stevan Z. Knezevic, University of Nebraska-LincolnFollow

ORCID IDs

Amit J. Jhala

Date of this Version

2017

Citation

Published in Pest Management Science (2017)

doi 10.1002/ps.4697

Comments

Copyright © 2017 Society of Chemical Industry; published by John Wiley Inc. Used by permission.

Abstract

Background: A population of Amaranthus tuberculatus (var. rudis) was confirmed resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitor herbicides (mesotrione, tembotrione, and topramezone) in a seed corn/soybean rotation in Nebraska. Further investigation confirmed a non-target-site resistance mechanism in this population. The main objective of this study was to explore the role of cytochrome P450 inhibitors in restoring the efficacy of HPPD-inhibitor herbicides on the HPPD-inhibitor resistant A. tuberculatus population from Nebraska, USA (HPPD-R).

Background: A population of Amaranthus tuberculatus (var. rudis) was confirmed resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitor herbicides (mesotrione, tembotrione, and topramezone) in a seed corn/soybean rotation in Nebraska. Further investigation confirmed a non-target-site resistance mechanism in this population. The main objective of this study was to explore the role of cytochrome P450 inhibitors in restoring the efficacy of HPPD-inhibitor herbicides on the HPPD-inhibitor resistant A. tuberculatus population from Nebraska, USA (HPPD-R).

Results: Enhanced metabolism via cytochrome P450 enzymes is the mechanism of resistance in HPPD-R. Amitrole partially restored the activity of mesotrione, whereas malathion, amitrole, and piperonyl butoxide restored the activity of tembotrione and topramezone in HPPD-R. Although corn was injured through malathion followed by mesotrione application a week after treatment, the injury was transient, and the crop recovered.

Includes supplementary file.