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A recent report from this laboratory described a method for isolation of low molecular weight compounds by chromatography on a pile of filter paper disks (1). Preliminary experiments have now been completed on the application of this method to the separation of enzymes. It was expected that a mixture of enzymes could be placed near the top of a filter paper pile and fractionated by imposing a gradient (i.e. pH, organic solvent, or salt concentration) on the column, thus taking advantage of a continuous process. Following the observation of Tiselius (2) it also seemed probable that enzyme separations could be obtained on the filter paper pile by salting-out adsorption and adsorption displacement.