Agronomy and Horticulture Department


Date of this Version



HortScience, 39(7):1688-1690. 2004.


Copyright 2004 American Society for Horticultural Science. Used by permission.


Microshoots of four hazelnut genotypes grown in vitro on Nas and Read medium (NRM) containing various combinations of CuSO4 5H2O and myo-inositol were successfully rooted and acclimatized ex vitro without any need of in vitro hardening treatments. Dipping of shoot bases in 1000 ppm indole-3-butyric acid (IBA) solution for 5 or 10 seconds followed by placement of shoots in plant growth regulator free NRM gave rise to formation of roots as early as 8 days. Shoots treated for 5 and 10 seconds rooted similarly, and depending on genotype, 88%-98% rooting was observed within 15 days after treatment with IBA.