Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase

Authors

Kohei Homma, University of Nebraska - Lincoln
Hideaki Moriyama, University of Nebraska - LincolnFollow

Date of this Version

2009

Citation

Acta Cryst. (2009). F65, 1030–1034

doi:10.1107/S1744309109034459

Comments

Open Access licensed.

Abstract

The 141-amino-acid deoxyuridine triphosphatase (dUTPase) from the algal Chlorella virus IL-3A and its Glu81Ser/Thr84Arg-mutant derivative Mu-22 were crystallized using the hanging-drop vapor-diffusion method at 298 K with polyethylene glycol as the precipitant. An apo IL-3A dUTPase with an aminoterminal T7 epitope tag and a carboxy-terminal histidine tag yielded cubic P213 crystals with unit-cell parameter a = 106.65 A . In the presence of dUDP, the enzyme produced thin stacked orthorhombic P222 crystals with unit-cell parameters a = 81.0, b = 96.2, c = 132.8 A. T7-histidine-tagged Mu-22 dUTPase formed thin stacked rectangular crystals. Amino-terminal histidine-tagged dUTPases did not crystallize but formed aggregates. Glycyl-seryl-tagged dUTPases yielded cubic P2₁3 IL-3A crystals with unit-cell parameter a = 105.68 A and hexagonal P6₃ Mu-22 crystals with unit-cell parameters a = 132.07, c = 53.45 A , ƴ = 120°. Owing to the Thr84Arg mutation, Mu-22 dUTPase had different monomer-to-monomer interactions to those of IL-3A dUTPase.