Integrating Evolutionary and Functional Tests of Adaptive Hypotheses: A Case Study of Altitudinal Differentiation in Hemoglobin Function in an Andean Sparrow, Zonotrichia capensis

Authors

Zachary A. Cheviron, University of Illinois at Urbana-ChampaignFollow
Chandrasekhar Natarajan, University of Nebraska-LincolnFollow
Joana Projecto-Garcia, University of Nebraska–LincolnFollow
Douglas K. Eddy, University of Illinois, Urbana- Champaign
Jennifer Jones, University of Illinois, Urbana- Champaign
Matthew D. Carling, University of Wyoming
Christopher C. Witt, University of New Mexico
Hideaki Moriyama, University of Nebraska-LincolnFollow
Roy E. Weber, Aarhus University, AarhusFollow
Angela Fago, Aarhus University
Jay F. Storz, University of Nebraska-LincolnFollow

Date of this Version

2014

Citation

Mol Biol Evol. 31(11): 2948-2962

DOI: 10.1093/molbev/msu234

Comments

© The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved.

Abstract

In air-breathing vertebrates, the physiologically optimal blood-O_² affinity is jointly determined by the prevailing partial pressure of atmospheric O₂, the efficacy of pulmonary O₂ transfer, and internal metabolic demands. Consequently, genetic variation in the oxygenation properties of hemoglobin (Hb) may be subject to spatially varying selection in species with broad elevational distributions. Here we report the results of a combined functional and evolutionary analysis of Hb polymorphism in the rufouscollared sparrow (Zonotrichia capensis), a species that is continuously distributed across a steep elevational gradient on the Pacific slope of the Peruvian Andes. We integrated a population genomic analysis that included all postnatally expressed Hb genes with functional studies of naturally occurring Hb variants, as well as recombinant Hb (rHb) mutants that were engineered through site-directed mutagenesis. We identified three clinally varying amino acid polymorphisms: Two in the α^A-globin gene, which encodes the α-chain subunits of the major HbA isoform, and one in the α^D-globin gene, which encodes the α-chain subunits of the minor HbD isoform. We then constructed and experimentally tested single- and double-mutant rHbs representing each of the alternative α^A-globin genotypes that predominate at different elevations. Although the locusspecific patterns of altitudinal differentiation suggested a history of spatially varying selection acting on Hb polymorphism, the experimental tests demonstrated that the observed amino acid mutations have no discernible effect on respiratory properties of the HbA or HbD isoforms. These results highlight the importance of experimentally validating the hypothesized effects of genetic changes in protein function to avoid the pitfalls of adaptive storytelling.