Complete Genome Sequence of Sulfolobus solfataricus Strain 98/2 and Evolved Derivatives

Authors

Samuel McCarthy, University of Nebraska-Lincoln
Julien Gradnigo, University of Nebraska-Lincoln
Tyler Johnson, University of Nebraska-Lincoln
Sophie Payne, University of Nebraska-LincolnFollow
Anna Lipzen, U.S. Department of Energy-Joint Genome Institute
Joel Martin, U.S. Department of Energy-Joint Genome Institute
Wendy Schackwitz, U.S. Department of Energy-Joint Genome Institute
Etsuko Morlyama, University of Nebraska-Lincoln
Paul H. Blum, University of Nebraska - LincolnFollow

Document Type

Article

Date of this Version

2015

Citation

McCarthy S, Gradnigo J, Johnson T, Payne S, Lipzen A, Martin J, Schackwitz W, Moriyama E, Blum P. 2015. Complete genome sequence of Sulfolobus solfataricus strain 98/2 and evolved derivatives. Genome Announc 3(3):e00549-15.

Comments

U.S. Government Work

Abstract

Sulfolobus solfataricus strain 98/2 is a thermoacidophilic chemoheterotrophic crenarcheote that grows optimally at 80°C and pH3.0 (1). The S. solfataricus 98/2 genome reported in 2009 (Gen- Bank accession no. CP001800.1, RefSeq NC_017274.1, GI: 261600703) is a deletion derivative of strain 98/2 called PBL2025 (2). It is often misconstrued as the wild-type strain 98/2, yet lacks a 50-kb region encoding numerous genes involved in sugar metabolism. A new closed and complete genome sequence for wildtype strain 98/2 referred to as SULA is presented here as GenBank accession no. CP011057 using locus tag SULA. This strain has been deposited at the Japan Collection of Microorganisms. Two additional closed and complete genomes derived from strain 98/2 are also presented and include SULB (GenBank accession no. CP011055) and SULC (GenBank accession no. CP011056). SULB and SULC resulted from extensive passage during selection for the biological trait of increased acid resistance (unpublished data).