Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana

Authors

Mamta Bajaj, University of Nebraska - Lincoln
Hideaki Moriyama, University of Nebraska at LincolnFollow

Date of this Version

March 2007

Comments

Published in Acta Cryst. (2007). F63, 409–411. Copyright © 2007 International Union of Crystallography. Used by permission.

Abstract

The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 Å resolution using Cu Kα radiation. The crystal belongs to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 69.90, b = 70.86 Å, c = 75.55 Å. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V_M of 1.8 ų Da^-1.