Purification of the Protein Crystal from Bacillus thuringiensis by Zonal Gradient Centrifugation

Authors

Kenneth W. Nickerson, University of Nebraska-LincolnFollow
Barabara J. Ang, University of Nebraska-Lincoln

Date of this Version

October 1978

Comments

Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Oct. 1978, p. 625-626. Copyright 1978 American Society for Microbiology. Used by permission.

Abstract

A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.