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The production of 2,3-butanediol from natural materials by fermentation offers a practical and economical method for obtaining appreciable quantities of this important chemical. A variety of substrates have been used satisfactorily such as wood hydrolyzates (Perlman,1944), wheat (Katznelson, 1944.; Ledingham et al., 1945; Ward et al., 1945) and corn (Ward et al., 1945). The use of several grades of beet molasses prepared from home-grown beets has been reported by British (Freeman and Morrison, 1947) and Canadian workers (Anastassiadis and Wheat, 1953). Excellent yields were obtained which compared favorably with those resulting from the use of pure sucrose. Although the characteristics of three distinct fermentations yielding this chemical have been determined (Freeman, 1947), of concern to us was the use of Aerobacter aerogenes since the fermentable carbohydrate in beet molasses is primarily sucrose. In this paper are presented the results of a study to determine the suitability of sugar beet molasses as a fermentable medium for the production of 2,3-butanediol.