Lipid Metabolism During Bacterial Growth, Sporulation, and Germination: an Obligate Nutritional Requirement in Bacillus thuringiensis for Compounds That Stimulate Fatty Acid Synthesis

Authors

Kenneth W. Nickerson, University of Nebraska-LincolnFollow
Lee A. Bulla Jr.

Date of this Version

August 1975

Comments

Published in JOURNAL OF BACTERIOLOGY, Aug. 1975, p. 598-603. Copyright © 1975 American Society for Microbiology. Used by permission.

Abstract

The regulation of fatty acid biosynthesis by compounds that are required for growth of Bacillus thuringiensis was investigated using an in vivo assay developed to measure fatty acid synthesis in germinating spores. A minimal glucoseammonium- salts medium does not support growth even though previous radiorespirometric studies have shown B. thuringiensis to possess intact tricarboxylic acid and Embden-Meyerhof-Parnas pathways. Abundant growth does occur, however, when this medium is supplemented with either glutamate, aspartate, citrate, thiosulfate, cystine, or ethylenediaminetetraacetic acid. Cells held under non-growing conditions incorporate acetate into fatty acids; fatty acid synthesis is stimulated by the compounds that permit growth. These alternate nutritional requirements are not manifestations of a vitamin or trace metal deficiency and do not reflect a chelation phenomenon. These results indicate a direct correlation between the capacity of these compounds to promote growth and to stimulate formation of fatty acids.