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Because of the relatively large sediment concentrations sometimes found on upland regions, adsorption of fluorescent dye onto sediment may be of concern. A laboratory study was conducted to identify the effects of sediment and dye concentration on adsorption. Sediment and dye concentration were both found to significantly affect adsorptive dye loss of rhodamine WT and sulpho rhodamine B.
Regression equations were developed which related dye adsorption to sediment and dye concentration. For a particular soil and dye material, regression equations may be used to correct for adsorptive dye loss. Sulpho rhodamine B is recommended as the dye of choice for the given experimental conditions.