Fast and efficient generation of influenza A virus like particles from synthetic genes

Authors

Theresa Schinko, University of Natural Resources and Applied Life Sciences, Vienna, AustriaFollow
Harutha Thaisuchat, University of Natural Resources and Applied Life Sciences, Vienna, AustriaFollow
Hendrik J. Viljoen, University of Nebraska - LincolnFollow
Nisha Padhye, University of Nebraska - Lincoln
Reingard Grabherr, University of Natural Resources and Applied Life Sciences, Vienna, AustriaFollow

Date of this Version

10-4-2006

Comments

Published in Microbial Cell Factories 2006, 5(Suppl 1):P39 doi:10.1186/1475-2859-5-S1-P39 Copyright © 2006 Schinko et al; licensee BioMed Central Ltd. Used by permission.
Poster presentation from The 4th Recombinant Protein Production Meeting: a comparative view on host physiology. Barcelona, Spain. 21–23 September 2006

Abstract

Co-transfection of bacmids resulted in the generation of influenza A virus like particles in the supernatant of Sf9 cells. VLPs were purified by means of Sucrose gradient centrifugation and the expected results were confirmed by Electron microscopy, Western Blot analysis and hemagglutination assays.