Structure Function Analysis of an ADP-ribosyltransferase Type III Effector and Its RNA-binding Target in Plant Immunity

Authors

Byeong-ryool Jeong, University of Nebraska-Lincoln
Yan Lin, Fudan University
Anna Joe, University of Nebraska-Lincoln
Ming Guo, University of Nebraska-Lincoln
Christin Korneli, University of Bielefeld
Huirong Yang, Fudan University
Ping Wang, Fudan University
Min Yu, Fudan University
Ronald Cerny, University of Nebraska - LincolnFollow
Dorothee Staiger, University of Bielefeld
James R. Alfano, University of Nebraska-LincolnFollow
Yanhui Xu, Fudan University

ORCID IDs

James R. Alfano

Date of this Version

2011

Citation

The Journal of Biological Chemistry VOL. 286, NO. 50, pp. 43272–43281, December 16, 2011

Comments

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

Abstract

Background: HopU1 ADP-ribosylates GRP7, suppressing plant immunity.

Results: The HopU1 structure has two novel loops required for GRP7 recognition, and HopU1 ribosylates GRP7 at an arginine in position 49 disrupting its function.

Conclusion: HopU1 targets a conserved arginine in GRP7, disabling its ability to bind immunity-related RNA.

Significance: The mechanistic details of how HopU1 recognizes its substrate reveal how HopU1 contributes to pathogenesis.