Off-campus UNL users: To download campus access dissertations, please use the following link to log into our proxy server with your NU ID and password. When you are done browsing please remember to return to this page and log out.
Non-UNL users: Please talk to your librarian about requesting this dissertation through interlibrary loan.
Studies of drug-protein interactions using high-performance affinity chromatography
Abstract
High-performance affinity chromatography (HPAC) is a type of liquid chromatography in which solutes are separated based on their binding to a stationary phase that is a biologically-related agent. Because of the strong and selective nature of many biological interactions, this method has already become a powerful technique for the purification and analysis of solutes that are complementary to the immobilized binding agent. Human serum albumin (HSA), the most abundant protein in the blood with concentrations of 35-50 mg/mL in serum, has interactions with many drugs, which can affect the absorption, distribution, metabolism and excretion of such agents. This dissertation describes the use of HPAC to examine the binding of various drugs to HSA and various forms of this protein. (Abstract shortened by ProQuest.)
Subject Area
Pharmacology|Biochemistry
Recommended Citation
Li, Zhao, "Studies of drug-protein interactions using high-performance affinity chromatography" (2016). ETD collection for University of Nebraska-Lincoln. AAI10143307.
https://digitalcommons.unl.edu/dissertations/AAI10143307